Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348849
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGrillo, Renato-
dc.contributor.authorunicampMelo, Nathalie Ferreira Silva de-
dc.contributor.authorunicampMoraes, Carolina Morales-
dc.contributor.authorunicampFraceto, Leonardo Fernandes-
dc.typeArtigopt_BR
dc.titleStudy of the interaction between hydroxymethylnitrofurazone and 2-hydroxypropyl-beta-cyclodextrinpt_BR
dc.contributor.authorGrillo, Renato-
dc.contributor.authorde Melo, Nathalie F. S.-
dc.contributor.authorMoraes, Carolina Morales-
dc.contributor.authorde Lima, Renata-
dc.contributor.authorMenezes, Carla M. S.-
dc.contributor.authorFerreira, Elizabeth Igne-
dc.contributor.authorRosa, Andre Henrique-
dc.contributor.authorFraceto, Leonardo Fernandes-
dc.subjectRessonância magnética nuclearpt_BR
dc.subjectHidroxipropil-beta-ciclodextrinapt_BR
dc.subject.otherlanguageHidroxypropyl-beta-cyclodextrinpt_BR
dc.subject.otherlanguageNuclear magnetic resonancept_BR
dc.description.abstractChagas disease is a serious health problem in Latin America. Hidroxymethylnitrofurazone (NFOH) is a nitrofurazone prodrug more active than nitrofurazone against Trypanosoma cruzi. However, NFOH presents low aqueous solubility, high photodecomposition and high toxicity. The present work is focused on the characterization of an inclusion complex of NFOH in 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD). The complexation with HP-beta-CD was investigated using reversed-phase liquid chromatography, solubility isotherms and nuclear magnetic resonance. The retention behavior was analyzed on a reversed-phase C-18 column, using acetonitrile-water (20/80, v/v) as the mobile phase, in which HP-beta-CD was incorporated as a mobile phase additive. The decrease in the retention times with increasing concentrations of HP-beta-CD enables the determination of the apparent stability constant of the complex (K = 6.2 +/- 0.3 M-1) by HPLC. The solubility isotherm was studied and the value for the apparent stability constant (K = 7.9 +/- 0.2 M-1) was calculated. The application of continuous variation method indicated the presence of a complex with 1:1 NFOH:HP-beta-CD stoichiometry. The photostability study showed that the formation of an inclusion complex had a destabilizing effect on the photodecomposition of NFOH when compared to that of the "free" molecule in solution. The mobility investigation (by NMR longitudinal relaxation time) gives information about the complexation of NFOH with HP-beta-CD. In preliminary toxicity studies, cell viability tests revealed that inclusion complexes were able to decrease the toxic effect (p < 0.01) caused by NFOHpt_BR
dc.relation.ispartofJournal of pharmaceutical and biomedical analysispt_BR
dc.relation.ispartofabbreviationJ pharm biomed analpt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2008-
dc.date.monthofcirculationJunept_BR
dc.language.isoengpt_BR
dc.description.volume47pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage295pt_BR
dc.description.lastpage302pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0731-7085pt_BR
dc.identifier.eissn1873-264Xpt_BR
dc.identifier.doi10.1016/j.jpba.2008.01.010pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S073170850800040Xpt_BR
dc.date.available2020-09-08T15:50:55Z-
dc.date.accessioned2020-09-08T15:50:55Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-09-08T15:50:55Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:52Z : No. of bitstreams: 1 000256649400010.pdf: 463720 bytes, checksum: 814d0b1a4e88273262acd4a223dd5aee (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-08T15:50:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2008en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348849-
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordSolubility isothermpt_BR
dc.identifier.source000256649400010pt_BR
dc.creator.orcid0000-0002-0284-5782pt_BR
dc.creator.orcid0000-0003-0765-4421pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-2827-2038pt_BR
dc.type.formArtigopt_BR
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000256649400010.pdf452.85 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.