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Type: Artigo
Title: Transdentinal protective role of sodium ascorbate against the cytopathic effects of H(2)O(2) released from bleaching agents
Author: Lima, Adriano Fonseca
Lessa, Fernanda Campos Rosetti
Mancini, Maria Nadir Gasparoto
Hebling, Josimeri
Costa, Carlos Alberto de Souza
Marchi, Giselle Maria
Abstract: The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Study design: Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. Results: In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. Conclusions: The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP.
Subject: Clareadores
Country: Estados Unidos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.tripleo.2009.12.020
Date Issue: 2010
Appears in Collections:FOP - Artigos e Outros Documentos

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