Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348817
Type: Artigo
Title: Essential role of nuclear factor-κB for NADPH oxidase activity in normal and anhidrotic ectodermal dysplasia leukocytes
Author: Luengo-Blanco, Marcos
Prando, Carolina
Bustamante, Jacinta
Arago Filho, Walmir Cutrim
Pereira, Paulo Vitor Soeiro
Rehder, Jussara
Padden, Carolyn
Casanova, Jean-Laurent
Newburger, Peter E.
Condino Neto, Antonio
Abstract: This work investigated the functional role of nuclear factor–κB (NF-κB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase genes CYBB, CYBA, NCF1, and NCF2. U937 cells with a stably transfected repressor of NF-κB (IκBα-S32A/S36A) demonstrated significantly lower superoxide release and lower CYBB and NCF1 gene expression compared with control U937 cells. We further tested Epstein-Barr virus (EBV)-transformed B cells from patients with anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID), an inherited disorder of NF-κB function. Superoxide release and CYBB gene expression by EDA-ID cells were significantly decreased compared with healthy cells and similar to cells from patients with X-linked chronic granulomatous disease (X910 CGD). NCF1 gene expression in EDA-ID S32I cells was decreased compared with healthy control cells and similar to that in autosomal recessive (A470) CGD cells. Gel shift assays demonstrated loss of recombinant human p50 binding to a NF-κB site 5′ to the CYBB gene in U937 cells treated with NF-κB inhibitors, repressor-transfected U937 cells, and EDA-ID patients' cells. Zymosan phagocytosis was not affected by transfection of U937 cells with the NF-κB repressor. These studies show that NF-κB is necessary for CYBB and NCF1 gene expression and activation of the phagocyte NADPH oxidase in this model system
Subject: Linfocitos B
Country: Estados Unidos
Editor: American Society of Hematology
Rights: Fechado
Identifier DOI: 10.1182/blood-2007-07-099267
Address: https://ashpublications.org/blood/article/112/4/1453/25352
Date Issue: 2008
Appears in Collections:FCM - Artigos e Outros Documentos

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