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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAizza, Lilian Cristina Baldon-
dc.contributor.authorunicampDornelas, Marcelo Carnier-
dc.typeArtigopt_BR
dc.titleIntercellular transport of epidermis‐expressed MADS domain transcription factors and their effect on plant morphology and floral transitionpt_BR
dc.contributor.authorUrbanus, Susan L.-
dc.contributor.authorMartinelli, Adriana P.-
dc.contributor.authorDinh, Q. D. (Peter)-
dc.contributor.authorAizza, Lilian C. B.-
dc.contributor.authorDornelas, Marcelo C.-
dc.contributor.authorAngenent, Gerco C.-
dc.contributor.authorImmink, Richard G. H.-
dc.subjectProteína verde fluorescentept_BR
dc.subject.otherlanguageGreen fluorescent proteinpt_BR
dc.description.abstractDuring the lifetime of an angiosperm plant various important processes such as floral transition, specification of floral organ identity and floral determinacy, are controlled by members of the MADS domain transcription factor family. To investigate the possible non‐cell‐autonomous function of MADS domain proteins, we expressed GFP‐tagged clones of AGAMOUS (AG), APETALA3 (AP3), PISTILLATA (PI) and SEPALLATA3 (SEP3) under the control of the MERISTEMLAYER1 promoter in Arabidopsis thaliana plants. Morphological analyses revealed that epidermal overexpression was sufficient for homeotic changes in floral organs, but that it did not result in early flowering or terminal flower phenotypes that are associated with constitutive overexpression of these proteins. Localisations of the tagged proteins in these plants were analysed with confocal laser scanning microscopy in leaf tissue, inflorescence meristems and floral meristems. We demonstrated that only AG is able to move via secondary plasmodesmata from the epidermal cell layer to the subepidermal cell layer in the floral meristem and to a lesser extent in the inflorescence meristem. To study the homeotic effects in more detail, the capacity of trafficking AG to complement the ag mutant phenotype was compared with the capacity of the non‐inwards‐moving AP3 protein to complement the ap3 mutant phenotype. While epidermal expression of AG gave full complementation, AP3 appeared not to be able to drive all homeotic functions from the epidermis, perhaps reflecting the difference in mobility of these proteinspt_BR
dc.relation.ispartofThe plant journalpt_BR
dc.publisher.cityChichesterpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherWileypt_BR
dc.date.issued2010-
dc.date.monthofcirculationJulypt_BR
dc.language.isoengpt_BR
dc.description.volume63pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage60pt_BR
dc.description.lastpage72pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0960-7412pt_BR
dc.identifier.eissn1365-313Xpt_BR
dc.identifier.doi10.1111/j.1365-313X.2010.04221.xpt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2010.04221.xpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2020-09-04T22:49:27Z-
dc.date.accessioned2020-09-04T22:49:27Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-09-04T22:49:27Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:47Z : No. of bitstreams: 1 000279406400005.pdf: 1437598 bytes, checksum: a9878333aab47293ddb18df44f19c1d2 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-04T22:49:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2010en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348796-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Vegetalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordMADSpt_BR
dc.subject.keywordL1 expressionpt_BR
dc.subject.keywordIntercellular transportpt_BR
dc.subject.keywordNon-cell-autonomous functionpt_BR
dc.subject.keywordMorphologypt_BR
dc.identifier.source000279406400005pt_BR
dc.creator.orcid0000-0003-0543-3545pt_BR
dc.creator.orcid0000-0002-6710-3050pt_BR
dc.type.formArtigopt_BR
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