Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348781
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGozzo, Fábio Cesar-
dc.contributor.authorunicampPilau, Eduardo Jorge-
dc.typeArtigopt_BR
dc.titleProteomic analysis of papaya fruit ripening using 2DE-DIGEpt_BR
dc.contributor.authorNogueira, Silvia Beserra-
dc.contributor.authorLabate, Carlos Alberto-
dc.contributor.authorGozzo, Fabio Cesar-
dc.contributor.authorPilau, Eduardo Jorge-
dc.contributor.authorLajolo, Franco Maria-
dc.contributor.authorNascimento, João Roberto Oliveira do-
dc.subjectProteômicapt_BR
dc.subject.otherlanguageProteomicspt_BR
dc.description.abstractPapayas have a very short green life as a result of their rapid pulp softening as well as their susceptibility to physical injury and mold growth. The ripening-related changes take place very quickly, and there is a continued interest in the reduction of postharvest losses. Proteins have a central role in biological processes, and differential proteomics enables the discrimination of proteins affected during papaya ripening. A comparative analysis of the proteomes of climacteric and pre-climacteric papayas was performed using 2DE-DIGE. Third seven proteins corresponding to spots with significant differences in abundance during ripening were submitted to MS analysis, and 27 proteins were identified and classified into six main categories related to the metabolic changes occurring during ripening. Proteins from the cell wall (alpha-galactosidase and invertase), ethylene biosynthesis (methionine synthase), climacteric respiratory burst, stress response, synthesis of carotenoid precursors (hydroxymethylbutenyl 4-diphosphate synthase, GcpE), and chromoplast differentiation (fibrillin) were identified. There was some correspondence between the identified proteins and the data from previous transcript profiling of papaya fruit, but new, accumulated proteins were identified, which reinforces the importance of differential proteomics as a tool to investigate ripening and provides potentially useful information for maintaining fruit quality and minimizing postharvest lossespt_BR
dc.relation.ispartofJournal of proteomicspt_BR
dc.relation.ispartofabbreviationJ. proteomicspt_BR
dc.publisher.cityAmsterdam pt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2012-
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume75pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage1428pt_BR
dc.description.lastpage1439pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1874-3919pt_BR
dc.identifier.eissn1876-7737pt_BR
dc.identifier.doi10.1016/j.jprot.2011.11.015pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1874391911006439pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumber2008/52447-0pt_BR
dc.date.available2020-09-04T20:19:51Z-
dc.date.accessioned2020-09-04T20:19:51Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-09-04T20:19:51Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:45Z : No. of bitstreams: 1 000300533600028.pdf: 1080349 bytes, checksum: 239fef3b2a7fd1ed0e5bee7070b4c531 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-04T20:19:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348781-
dc.contributor.departmentDepartamento de Química Orgânicapt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordPapaya ripeningpt_BR
dc.subject.keywordPapaya proteomept_BR
dc.subject.keyword2DE-DIGEpt_BR
dc.subject.keywordGcpEpt_BR
dc.subject.keywordFibrillinpt_BR
dc.subject.keywordMEP pathwaypt_BR
dc.identifier.source000300533600028pt_BR
dc.creator.orcid0000-0002-5270-4427pt_BR
dc.creator.orcid0000-0002-9175-3520pt_BR
dc.type.formArtigopt_BR
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