Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348780
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampFerreira, Anna Laura Bechara Jacob-
dc.contributor.authorunicampPalei, Ana Carolina Taveiros-
dc.contributor.authorunicampMoreno Junior, Heitor-
dc.contributor.authorunicampGerlach, Raquel Fernanda-
dc.contributor.authorunicampTanus-Santos, Jose Eduardo-
dc.typeArtigopt_BR
dc.titleEvidence for the involvement of matrix metalloproteinases in the cardiovascular effects produced by nicotinept_BR
dc.contributor.authorJacob-Ferreira, Anna L. B.-
dc.contributor.authorPalei, Ana C. T.-
dc.contributor.authorCau, Stefany B.-
dc.contributor.authorMoreno Junior, Heitor-
dc.contributor.authorMartinez, Marcio L. L.-
dc.contributor.authorIzidoro-Toledo, Tatiane C.-
dc.contributor.authorGerlach, Raquel F.-
dc.contributor.authorTanus-Santos, Jose E.-
dc.subjectDisfunção endotelialpt_BR
dc.subjectNicotinapt_BR
dc.subject.otherlanguageEndothelial dysfunctionpt_BR
dc.subject.otherlanguageNicotinept_BR
dc.description.abstractNicotine plays a role in smoking-associated cardiovascular diseases, and may upregulate matrix metalloproteinase (MMP)-2 and MMP-9. We examined whether nicotine induces the release of MMP-2 and MMP-9 by rat smooth muscle cells (SMC), and whether doxycycline (non-selective MMP inhibitor) inhibits the vascular effects produced by nicotine. SMC were incubated with nicotine 0, 50, and 150 nM for 48 h. MMP-2 and MMP-9 levels in the cell supernatants were determined by gelatin zymography. The acute changes in mean arterial pressure caused by nicotine 2 mu mol/kg (or saline) were assessed in rats pretreated with doxycycline (or saline). We also examined whether doxcycline (30 mg/Kg, i.p., daily) modifies the effects of nicotine (10 mg/kg/day; 4 weeks) on the endothelium-dependent relaxations of rat aortic rings. Aortic MMP-2 levels were assessed by gelatin zymography. Aortic gelatinolytic activity was assessed using a gelatinolytic activity kit. MMP-2 and MMP-9 levels increased in the supernatant of SMC cells incubated with nicotine 150 nM (P<0.05) but not with 50 nM. Nicotine (2 mu mol/kg) produced lower increases in the mean arterial pressure in rats pretreated with doxycycline than those found in rats pretreated with saline (26 +/- 4 vs. 37 +/- 4 mmHg, respectively; P<0.05). Nicotine impaired of the endothelium-dependent responses to acetylcholine, and treatment with doxycycline increased the potency (pD2) by approximately 25% (P<0.05). While we found no significant differences in aortic MMP-2 levels, nicotine significantly increased gelatinolytic activity (P<0.05). These findings suggest that nicotine produces cardiovascular effects involving MMPs. It is possible that MMPs inhibition may counteract the effects produced by nicotinept_BR
dc.relation.ispartofEuropean journal of pharmacologypt_BR
dc.relation.ispartofabbreviationEur. j. pharmacol.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2010-
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume627pt_BR
dc.description.issuenumber1-3pt_BR
dc.description.firstpage216pt_BR
dc.description.lastpage222pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0014-2999pt_BR
dc.identifier.eissn1879-0712pt_BR
dc.identifier.doi10.1016/j.ejphar.2009.10.057pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0014299909009649pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.date.available2020-09-04T20:06:21Z-
dc.date.accessioned2020-09-04T20:06:21Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-09-04T20:06:21Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:45Z : No. of bitstreams: 1 000274093800032.pdf: 715123 bytes, checksum: 5b4fe3bb3bdc2cd8078576de241ba648 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-04T20:06:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2010en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348780-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Clínica Médicapt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordEndothelial dysfunctionpt_BR
dc.identifier.source000274093800032pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-6330-697Xpt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-9945-9633pt_BR
dc.type.formArtigopt_BR
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