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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampZecchin, Karina Gottardello-
dc.contributor.authorunicampVercesi, Anibal Eugenio-
dc.contributor.authorunicampGraner, Edgard-
dc.contributor.authorunicampCatharino, Rodrigo Ramos-
dc.typeArtigopt_BR
dc.titleVisualizing inhibition of fatty acid synthase through mass spectrometric analysis of mitochondria from melanoma cellspt_BR
dc.contributor.authorZecchin, K.G.-
dc.contributor.authorAlberici, L.C.-
dc.contributor.authorRiccio, M.F.-
dc.contributor.authorEberlin, M.N.-
dc.contributor.authorVercesi, A.E.-
dc.contributor.authorGraner, E.-
dc.contributor.authorCatharino, R.R.-
dc.subjectÁcidos graxospt_BR
dc.subject.otherlanguageFatty acidspt_BR
dc.description.abstractFatty acid synthase (FASN) is the metabolic enzyme responsible for the endogenous synthesis of the saturated long-chain fatty acid palmitate. In contrast to most normal cells, FASN is overexpressed in a variety of human cancers including cutaneous melanoma, in which its levels of expression are associated with a poor prognosis and depth of invasion. Recently, we have demonstrated the mitochondrial involvement in FASN inhibition-induced apoptosis in melanoma cells. Herein we compare, via electrospray ionization mass spectrometry (ESI-MS), free fatty acids (FFA) composition of mitochondria isolated from control (EtOH-treated cells) and Orlistat-treated B16-F10 mouse melanoma cells. Principal component analysis (PCA) was applied to the ESI-MS data and found to separate the two groups of samples. Mitochondria from control cells showed predominance of six ions, that is, those of m/z 157 (Pelargonic, 9:0), 255 (Palmitic, 16:0), 281 (Oleic, 18:1), 311 (Arachidic, 20:0), 327 (Docosahexaenoic, 22:6) and 339 (Behenic, 22:0). In contrast, FASN inhibition with Orlistat changes significantly mitochondrial FFA composition by reducing synthesis of palmitic acid, and its elongation and unsaturation products, such as arachidic and behenic acids, and oleic acid, respectively. ESI-MS of mitochondria isolated from Orlistat-treated cells presented therefore three major ions of m/z 157 (Pelargonic, 9:0), 193 (unknown) and 199 (Lauric, 12:0). These findings demonstrate therefore that FASN inhibition by Orlistat induces significant changes in the FFA composition of mitochondria.pt_BR
dc.relation.ispartofRapid communications in mass spectrometrypt_BR
dc.relation.ispartofabbreviationRapid commun mass spectrompt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherJohn Wiley & Sonspt_BR
dc.date.issued2011-
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume25pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage449pt_BR
dc.description.lastpage452pt_BR
dc.rightsFechadopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn0951-4198pt_BR
dc.identifier.eissn1097-0231pt_BR
dc.identifier.doi10.1002/rcm.4875pt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1002/rcm.4875pt_BR
dc.date.available2020-09-04T19:29:53Z-
dc.date.accessioned2020-09-04T19:29:53Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2020-09-04T19:29:53Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:45Z : No. of bitstreams: 1 2-s2.0-78651290740.pdf: 224132 bytes, checksum: 28c7e1b73eb51977fd02a664fec92b54 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-04T19:29:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2011en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348772-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Patologia Clínicapt_BR
dc.contributor.departmentDepartamento de Diagnóstico Oralpt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordAlcoholpt_BR
dc.subject.keywordFatty acid synthasept_BR
dc.subject.keywordlactonept_BR
dc.subject.keywordTetrahydrolipstatinpt_BR
dc.identifier.source2-s2.0-78651290740pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-0504-5676pt_BR
dc.creator.orcid0000-0002-4737-7935pt_BR
dc.creator.orcid0000-0001-7219-2644pt_BR
dc.type.formArtigopt_BR
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