Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348746
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampSouza, Claudio Teodoro de-
dc.contributor.authorunicampAraujo, Eliana Pereira de-
dc.contributor.authorunicampMorari, Joseane-
dc.contributor.authorunicampAlvarez Rojas, Fernanda-
dc.contributor.authorunicampMoreira Filho, Djalma de Carvalho-
dc.contributor.authorunicampCarvalheira, José Barreto Campello-
dc.contributor.authorunicampSaad, Mario José Abdalla-
dc.contributor.authorunicampVelloso, Licio Augusto-
dc.typeArtigopt_BR
dc.titleDistinct subsets of hypothalamic genes are modulated by two different thermogenesis‐inducing stimulipt_BR
dc.contributor.authorSouza, Cláudio T. De-
dc.contributor.authorSilva, Márcio Pereira‐da‐-
dc.contributor.authorAraujo, Eliana P.-
dc.contributor.authorMorari, Joseane-
dc.contributor.authorAlvarez‐Rojas, Fernanda-
dc.contributor.authorBordin, Silvana-
dc.contributor.authorMoreira‐Filho, Djalma C.-
dc.contributor.authorCarvalheira, José B.-
dc.contributor.authorSaad, Mario J.-
dc.contributor.authorVelloso, Licio A.-
dc.subjectTermogênesept_BR
dc.subject.otherlanguageThermogenesispt_BR
dc.description.abstractObesity results from an imbalance between food intake and energy expenditure, two vital functions that are tightly controlled by specialized neurons of the hypothalamus. The complex mechanisms that integrate these two functions are only beginning to be deciphered. The objective of this study was to determine the effect of two thermogenesis‐inducing conditions, i.e., ingestion of a high‐fat (HF) diet and exposure to cold environment, on the expression of 1,176 genes in the hypothalamus of Wistar rats. Hypothalamic gene expression was evaluated using a cDNA macroarray approach. mRNA and protein expressions were determined by reverse‐transcription PCR (RT‐PCR) and immunoblot. Cold exposure led to an increased expression of 43 genes and to a reduced expression of four genes. HF diet promoted an increased expression of 90 genes and a reduced expression of 78 genes. Only two genes (N‐methyl‐d‐aspartate (NMDA) receptor 2B and guanosine triphosphate (GTP)‐binding protein G‐alpha‐i1) were similarly affected by both thermogenesis‐inducing conditions, undergoing an increment of expression. RT‐PCR and immunoblot evaluations confirmed the modulation of NMDA receptor 2B and GTP‐binding protein G‐alpha‐i1, only. This corresponds to 0.93% of all the responsive genes and 0.17% of the analyzed genes. These results indicate that distinct environmental thermogenic stimuli can modulate predominantly distinct profiles of genes reinforcing the complexity and multiplicity of the hypothalamic mechanisms that regulate energy conservation and expenditurept_BR
dc.relation.ispartofObesity : a research journalpt_BR
dc.publisher.cityHoboken, NJpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherWileypt_BR
dc.date.issued2008-
dc.date.monthofcirculationJunept_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage1239pt_BR
dc.description.lastpage1247pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1930-7381pt_BR
dc.identifier.eissn1930-739Xpt_BR
dc.identifier.doi10.1038/oby.2008.53pt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1038/oby.2008.53pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.date.available2020-09-04T17:20:17Z-
dc.date.accessioned2020-09-04T17:20:17Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-09-04T17:20:17Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:42Z : No. of bitstreams: 1 000256353700012.pdf: 249815 bytes, checksum: d1f4bbdbd3353045ca3d6dea7bebca7a (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-04T17:20:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2008en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348746-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Saúde Coletivapt_BR
dc.contributor.departmentDepartamento de Clínica Médicapt_BR
dc.contributor.departmentDepartamento de Clínica Médicapt_BR
dc.contributor.departmentDepartamento de Clínica Médicapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordHypothalamicpt_BR
dc.identifier.source000256353700012pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-7539-8477pt_BR
dc.creator.orcid0000-0002-0728-4283pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-7943-0868pt_BR
dc.creator.orcid0000-0002-0136-0943pt_BR
dc.creator.orcid0000-0003-4544-6105pt_BR
dc.creator.orcid0000-0002-4806-7218pt_BR
dc.type.formArtigopt_BR
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