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|Title:||Cramoll 1,4 lectin increases ROS production, calcium levels, and cytokine expression in treated spleen cells of rats|
|Author:||Melo, Cristiane Moutinho Lagos de|
Paim, Bruno Alves
Zecchin, Karina Gotardelo
Chiaratti, Marcos R.
Correia, Maria Tereza Santos
Coelho, Luana Cassandra B. Barroso
Paiva, Patrícia Maria Guedes
|Abstract:||This study reports the in vivo stimulatory effects of Cramoll 1,4 on rat spleen lymphocytes as evidenced by an increase in intracellular reactive oxygen species (ROS) production, Ca2+ levels, and interleukin (IL)-1β expression. Cramoll 1,4 extracted from seeds of the Leguminosae Cratylia mollis Mart., is a lectin with antitumor and lymphocyte mitogenic activities. Animals (Nine-week-old male albino Wistar rats, Rattus norvegicus) were treated with intraperitoneal injection of Cramoll 1,4 (235 μg ml−1 single dose) and, 7 days later, spleen lymphocytes were isolated and analyzed for intracellular ROS, cytosolic Ca2+, and IL-6, IL-10, and IL-1 mRNAs. Cell viability was investigated by annexin V-FITC and 7-amino-actinomycin D staining. The data showed that in lymphocytes activated by Cramoll 1,4 the increase in cytosolic and mitochondrial ROS was related to higher cytosolic Ca2+ levels. Apoptosis and necrosis were not detected in statistically significant values and thus the lectin effector activities did not induce lymphocyte death. In vivo Cramoll 1,4 treatment led to a significant increase in IL-1β but IL-6 and -10 levels did not change. Cramoll 1,4 had modulator activities on spleen lymphocytes and stimulated the Th2 response|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
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