Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348701
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampZanella, Cesar Augusto Bueno-
dc.contributor.authorunicampZucchi, Fabiola Cristina Ribeiro-
dc.contributor.authorunicampDe Nucci, Gilberto-
dc.typeArtigopt_BR
dc.titleImmunolocalization of nitric oxide synthase isoforms in human archival and rat tissues, and cultured cellspt_BR
dc.contributor.authorMartins, Antonio R-
dc.contributor.authorZanella, Cesar A. B.-
dc.contributor.authorZucchi, Fabiola C. R.-
dc.contributor.authorDombroski, Thais C. D.-
dc.contributor.authorCosta, Edmar T.-
dc.contributor.authorGuethe, Liliane M.-
dc.contributor.authorOliveira, Alina O.-
dc.contributor.authorDonatti, Ana L. F.-
dc.contributor.authorNeder, Luciano-
dc.contributor.authorChimelli, Leila-
dc.contributor.authorDe Nucci, Gilberto-
dc.contributor.authorLee-Ho, Paulo-
dc.contributor.authorMurad, Ferid-
dc.subjectÓxido nítrico sintasept_BR
dc.subjectImuno-histoquímicapt_BR
dc.subject.otherlanguageNitric oxide synthasept_BR
dc.subject.otherlanguageImmunohistochemistrypt_BR
dc.description.abstractNitric oxide (NO) exerts important physiological and pathological roles in humans. The study of NO requires the immunolocalization of its synthesizing enzymes, neuronal, endothelial and inducible NO synthases (NOS). NOS are labile to formalin-fixation and paraffin-embedding, which are used to prepare human archival tissues. This lability has made NOS immunohistochemical studies difficult, and a detailed protocol is not yet available. We describe here a protocol for the immunolocalization of NOS isoforms in human archival cerebellum and non-nervous tissues, and in rat tissues and cultured cells. Neuronal NOS antigenicity in human archival and rat nervous tissue sections was microwave-retrieved in 50 mM Tris-HCl buffer, pH 9.5, for 20 min at 900W. Neuronal NOS was expressed in stellate, basket, Purkinje and granule cells in human and rat cerebellum. Archival and frozen human cerebellar sections showed the same neuronal NOS staining pattern. Archival cerebellar sections not subjected to antigen retrieval stained weakly. Antigenicity of inducible NOS in human lung was best retrieved in 10 mM sodium citrate buffer, pH 6.0, for 15 min at 900W. Inflammatory cells in a human lung tuberculoma were strongly stained by anti-inducible NOS antibody. Anti-endothelial NOS strongly stained kidney glomeruli. Cultured PC12 cells were strongly stained by anti-neuronal NOS without antigen retrieving. The present immunohistochemistry protocol is easy to perform, timeless, and suitable for the localization of NOS isoforms in nervous and non-nervous tissues, in human archival and rat tissues. It has been extensively used in our laboratory, and is also appropriate for other antigenspt_BR
dc.relation.ispartofJournal of neuroscience methodspt_BR
dc.relation.ispartofabbreviationJ. neurosci. methodspt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2011-
dc.date.monthofcirculationMaypt_BR
dc.language.isoengpt_BR
dc.description.volume198pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage16pt_BR
dc.description.lastpage22pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0165-0270pt_BR
dc.identifier.eissn1872-678Xpt_BR
dc.identifier.doi10.1016/j.jneumeth.2011.02.024pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0165027011001191pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.date.available2020-09-04T12:42:26Z-
dc.date.accessioned2020-09-04T12:42:26Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-09-04T12:42:26Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:37Z : No. of bitstreams: 1 000291142600003.pdf: 902256 bytes, checksum: a4fb9c0100247bfbaa33402439bef7a2 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-04T12:42:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2011en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348701-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Farmacologiapt_BR
dc.contributor.unidadeFaculdade de Ciências Mèdicaspt_BR
dc.contributor.unidadeFaculdade de Ciencias Medicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordLungpt_BR
dc.subject.keywordHuman and rat nervous systempt_BR
dc.identifier.source000291142600003pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-2252-613Xpt_BR
dc.creator.orcid0000-0002-4346-7941pt_BR
dc.type.formArtigopt_BR
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