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dc.contributor.authorunicampPonce-Soto, Luis Alberto-
dc.contributor.authorunicampMarangoni, Sergio-
dc.contributor.authorunicampDal Belo, Cháriston André-
dc.contributor.authorunicampHyslop, Stephen-
dc.contributor.authorunicampSimioni, Lea Rodrigues-
dc.titleNeuromuscular activity of BaTX, a presynaptic basic PLA(2) isolated from Bothrops alternatus snake venompt_BR
dc.contributor.authorPonce-Soto, L. A.-
dc.contributor.authorBarros, J. C.-
dc.contributor.authorMarangoni, S.-
dc.contributor.authorHernandez, S.-
dc.contributor.authorDal Belo, C. A.-
dc.contributor.authorCorrado, A. P.-
dc.contributor.authorHyslop, S.-
dc.contributor.authorRodrigues-Simioni, L.-
dc.subjectVenenos elapídicospt_BR
dc.subjectBloqueio neuromuscularpt_BR
dc.subject.otherlanguageElapid Venomspt_BR
dc.subject.otherlanguageNeuromuscular blockadept_BR
dc.description.abstractWe have previously isolated a Lys49 phospholipase A(2) homolog (BaTX) from Bothrops alternatus snake venom using a combination of molecular exclusion chromatography and reverse phase HPLC and shown its ability to cause neuromuscular blockade. In this work, we describe a one-step procedure for the purification of this toxin and provide further details of its neuromuscular activity. The toxin was purified by reverse phase HPLC and its purity and molecular mass were confirmed by SIDS-PAGE, MALDI-TOF mass spectrometry, amino acid analysis and N-terminal sequencing. BaTX (0.007-1.4 mu M) produced time-dependent, irreversible neuromuscular blockade in isolated mouse phrenic nerve-diaphragm and chick biventer cervicis preparations (time to 50% blockade with 0.35 mu M toxin: 58 +/- 4 and 24 +/- 1 min, respectively; n = 3-8; mean +/- S.E.) without significantly affecting the response to direct muscle stimulation. In chick preparations, contractures to exogenous acetylcholine (55 and 110 mu M) or KCl (13.4 mM) were unaltered after complete blockade by all toxin concentrations. These results, which strongly suggested a presynaptic mechanism of action for this toxin, were reinforced by (1) the inability of BaTX to interfere with the carbachol-induced depolarization of the resting membrane, (2) a significant decrease in the frequency and amplitude of miniature end-plate potentials, and (3) a significant reduction (59 +/- 4%, n=12) in the quantal content of the end-plate potentials after a 60 min incubation with the toxin (1.4 mu M). In addition, a decrease in the organ bath temperature from 37 degrees C to 24 degrees C and/or the replacement of calcium with strontium prevented the neuromuscular blockade, indicating a temperature-dependent effect possibly mediated by enzymatic activitypt_BR
dc.relation.ispartofComparative biochemistry and physiology. Part C: toxicology and pharmacologypt_BR
dc.relation.ispartofabbreviationComp biochem physiol C toxicol pharmacolpt_BR
dc.publisher.cityPhiladelphia, PApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.description.provenanceSubmitted by Cintia Oliveira de Moura ( on 2020-09-03T15:28:39Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:31Z : No. of bitstreams: 1 000268206600022.pdf: 783032 bytes, checksum: fcc67ee8118f34bdb21a0615ecf82d8b (MD5)en
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dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Farmacologiapt_BR
dc.contributor.departmentDepartamento de Farmacologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordPresynaptic PLA(2)pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
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