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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampHuancahuire-Vega, Salomón-
dc.contributor.authorunicampMarangoni, SergioDepartamento de Bioquímica e Biologia Tecidual-
dc.typeArtigopt_BR
dc.titleCrystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A2 from Bothrops brazili venompt_BR
dc.contributor.authorFernandes, Carlos A. H.-
dc.contributor.authorGartuzo, Elaine C. G.-
dc.contributor.authorPagotto, Ivan-
dc.contributor.authorComparetti, Edson J.-
dc.contributor.authorHuancahuire-Vega, Salomon-
dc.contributor.authorPonce-Soto, Luis Alberto-
dc.contributor.authorCosta, Tassia R.-
dc.contributor.authorMarangoni, Sergio-
dc.contributor.authorSoares, Andreimar M.-
dc.contributor.authorFontes, Marcos R. M.-
dc.subjectFosfolipases A2pt_BR
dc.subject.otherlanguagePhospholipases A2pt_BR
dc.description.abstractTwo myotoxic and noncatalytic Lys49-phospholipases A2 (braziliantoxin-II and MT-II) and a myotoxic and catalytic phospholipase A2 (braziliantoxin-III) from the venom of the Amazonian snake Bothrops brazili were crystallized. The crystals diffracted to resolutions in the range 2.562.05 angstrom and belonged to space groups P3121 (braziliantoxin-II), P6522 (braziliantoxin-III) and P21 (MT-II). The structures were solved by molecular-replacement techniques. Both of the Lys49-phospholipases A2 (braziliantoxin-II and MT-II) contained a dimer in the asymmetric unit, while the Asp49-phospholipase A2 braziliantoxin-III contained a monomer in its asymmetric unit. Analysis of the quaternary assemblies of the braziliantoxin-II and MT-II structures using the PISA program indicated that both models have a dimeric conformation in solution. The same analysis of the braziliantoxin-III structure indicated that this protein does not dimerize in solution and probably acts as a monomer in vivo, similar to other snake-venom Asp49-phospholipases A2pt_BR
dc.relation.ispartofActa crystallographica section F: sructural biology communicationspt_BR
dc.relation.ispartofabbreviationActa crystallogr. sect. F struct. biol. commun.pt_BR
dc.publisher.cityHoboken, NJpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherWiley-Blackwellpt_BR
dc.date.issued2012-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume68pt_BR
dc.description.issuepart8pt_BR
dc.description.firstpage935pt_BR
dc.description.lastpage938pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn2053-230Xpt_BR
dc.identifier.doi10.1107/S1744309112026073pt_BR
dc.identifier.urlhttp://scripts.iucr.org/cgi-bin/paper?S1744309112026073pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2020-09-01T18:25:36Z-
dc.date.accessioned2020-09-01T18:25:36Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-09-01T18:25:36Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:53Z : No. of bitstreams: 1 000307217700019.pdf: 366392 bytes, checksum: bb5e08f40e53109add76bcd66a34944d (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-01T18:25:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348482-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordBothrops brazilipt_BR
dc.subject.keywordBraziliantoxin-IIpt_BR
dc.subject.keywordBraziliantoxin-IIIpt_BR
dc.subject.keywordMT-IIpt_BR
dc.identifier.source000307217700019pt_BR
dc.creator.orcid0000-0002-4848-4767pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.type.formArtigopt_BR
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