Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348472
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampRonilson Agnaldo Moreno-
dc.contributor.authorunicampSverdloff, Carlos Eduardo-
dc.contributor.authorunicampBorges, Ney Carter do Carmo-
dc.typeArtigopt_BR
dc.titleDetermination of chlorpheniramine in human plasma by HPLC‐ESI‐MS/MS: application to a dexchlorpheniramine comparative bioavailability studypt_BR
dc.contributor.authorMoreno, Ronilson Agnaldo-
dc.contributor.authorOliveira‐Silva, Diogo-
dc.contributor.authorSverdloff, Carlos Eduardo-
dc.contributor.authorBorges, Bruno Carter-
dc.contributor.authorGalvinas, Paulo Alexandre Rebelo-
dc.contributor.authorAstigarraga, Rafael Barrientos-
dc.contributor.authorBorges, Ney Carter-
dc.subjectBiodisponibilidadept_BR
dc.subject.otherlanguageBioavailabilitypt_BR
dc.description.abstractIn the present study a fast, sensitive and robust validated method to quantify chlorpheniramine in human plasma using brompheniramine as internal standard (IS) is described. The analyte and the IS were extracted from plasma by LLE (diethyl ether–dichloromethane, 80:20, v/v) and analyzed by HPLC‐ESI‐MS/MS. Chromatographic separation was performed using a gradient of methanol from 35 to 90% with 2.5 mm NH4OH on a Gemini Phenomenex C8 5 μm column (50 × 4.6 mm i.d.) in 5.0 min/run. The method fitted to a linear calibration curve (0.05–10 ng/mL, R > 0.9991). The precision (%CV) and accuracy ranged, respectively: intra‐batch from 1.5 to 6.8% and 99.1 to 106.6%, and inter‐batch from 2.4 to 9.0%, and 99.9 to 103.1%. The validated bioanalytical procedure was used to assess the comparative bioavailability in healthy volunteers of two dexchlorpheniramine 2.0 mg tablet formulations (test dexchlorpheniramine, Eurofarma, and reference Celestamine®, Schering‐Plough). The study was conducted using an open, randomized, two‐period crossover design with a 2 week washout interval. Since the 90% confidence interval for Cmax and AUC ratios were all within the 80–125% interval proposed by ANVISA and FDA, it was concluded that test and reference formulations are bioequivalent concerning the rate and the extent of absorptionpt_BR
dc.relation.ispartofBiomedical chromatographypt_BR
dc.relation.ispartofabbreviationBiomed. chromatogr.pt_BR
dc.publisher.cityChichesterpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherJohn Wiley & Sonspt_BR
dc.date.issued2009-
dc.date.monthofcirculationNov.pt_BR
dc.language.isoengpt_BR
dc.description.volume24pt_BR
dc.description.issuenumber7pt_BR
dc.description.firstpage774pt_BR
dc.description.lastpage781pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1099-0801pt_BR
dc.identifier.doi10.1002/bmc.1362pt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.1362pt_BR
dc.date.available2020-09-01T17:28:18Z-
dc.date.accessioned2020-09-01T17:28:18Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-09-01T17:28:18Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:53Z : No. of bitstreams: 1 000279367900014.pdf: 482433 bytes, checksum: fad2989ce01246975caf7c92318049f0 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-01T17:28:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348472-
dc.contributor.departmentDepartamento de Farmacologiapt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordDexchlorpheniraminept_BR
dc.identifier.source000279367900014pt_BR
dc.creator.orcid0000-0001-6692-1011pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigo de pesquisapt_BR
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