Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348467
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampZorzeto, Tatiane Queiroz-
dc.contributor.authorunicampSilva, Marcos Tadeu Nolasco da-
dc.contributor.authorunicampCarniel, Emilia de Faria-
dc.contributor.authorunicampMorcillo, André Moreno-
dc.contributor.authorunicampAntonio, Maria Ângela Reis de Góes Monteiro-
dc.contributor.authorunicampZanolli, Maria de Lurdes-
dc.contributor.authorunicampVilela, Maria Marluce dos Santos-
dc.typeArtigopt_BR
dc.titleImmunogenicity of a whole-cell pertussis vaccine with low lipopolysaccharide content in infantspt_BR
dc.contributor.authorZorzeto, Tatiane Queiroz-
dc.contributor.authorHigashi, Hisako Gondo-
dc.contributor.authorSilva, Marcos Tadeu Nolasco da-
dc.contributor.authorCarniel, Emilia de Faria-
dc.contributor.authorDias, Waldely Oliveira-
dc.contributor.authorRamalho, Vanessa Domingues-
dc.contributor.authorMazzola, Taís Nitsch-
dc.contributor.authorLima, Simone Corte Batista Souza-
dc.contributor.authorMorcillo, André Moreno-
dc.contributor.authorStephano, Marco Antonio-
dc.contributor.authorAntonio, Maria Ângela Reis de Góes-
dc.contributor.authorZanolli, Maria de Lurdes-
dc.contributor.authorRaw, Isaias-
dc.contributor.authorVilela, Maria Marluce dos Santos-
dc.subjectLipopolissacarídeospt_BR
dc.subjectCélulaspt_BR
dc.subject.otherlanguageCellspt_BR
dc.subject.otherlanguageLipopolysaccharidespt_BR
dc.description.abstractThe lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wPlow vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wPlow vaccine. Proliferation of CD3 T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3 , CD4 , CD8 , and T-cell receptor -positive ( ) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in supernatants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3 blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wPlow vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wPlow vaccine; P 0.029). The frequencies of proliferating CD4 , CD8 , and cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of cells in the B. pertussis cultures (P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3 cell proliferation, and cell expansions were similar with the two vaccinespt_BR
dc.relation.ispartofClinical and vaccine immunologypt_BR
dc.relation.ispartofabbreviationClin. vaccine immunol.pt_BR
dc.publisher.cityWashington, DCpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherAmerican Society for Microbiologypt_BR
dc.date.issued2009-
dc.date.monthofcirculationApr.pt_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage544pt_BR
dc.description.lastpage550pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1556-6811pt_BR
dc.identifier.eissn1556-679Xpt_BR
dc.identifier.doi10.1128/CVI.00339-08pt_BR
dc.identifier.urlhttps://cvi.asm.org/content/16/4/544pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2005/03539-1pt_BR
dc.date.available2020-09-01T17:01:48Z-
dc.date.accessioned2020-09-01T17:01:48Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-09-01T17:01:48Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:52Z : No. of bitstreams: 1 000264938400017.pdf: 541849 bytes, checksum: d8081954dd8497821d3f7f79e9734a2f (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-01T17:01:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348467-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Pediatriapt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Pediatriapt_BR
dc.contributor.departmentDepartamento de Pediatriapt_BR
dc.contributor.departmentDepartamento de Pediatriapt_BR
dc.contributor.departmentDepartamento de Pediatriapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordImmunogenicitypt_BR
dc.identifier.source000264938400017pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0001-8342-1959pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-2088-972Xpt_BR
dc.creator.orcid0000-0002-7290-7950pt_BR
dc.creator.orcid0000-0001-7419-8257pt_BR
dc.creator.orcid0000-0002-2618-0148pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.description.sponsorNoteThis study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, São Paulo, Brazil, grant number 2005/03539-1, and by Financiadora de Estudos e Projetos, Brazil, grant number 01040957/0pt_BR
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