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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampMelo, Nathalie Ferreira Silva de-
dc.contributor.authorunicampFraceto, Leonardo Fernandes-
dc.typeArtigopt_BR
dc.titleExogenous administration of 15d-PGJ2–loaded nanocapsules inhibits bone resorption in a mouse periodontitis modelpt_BR
dc.contributor.authorNapimoga, Marcelo H.-
dc.contributor.authorda Silva, Carlos A. T.-
dc.contributor.authorCarregaro, Vanessa-
dc.contributor.authorFarnesi-de-Assuncao, Thais S.-
dc.contributor.authorDuarte, Poliana M.-
dc.contributor.authorde Melo, Nathalie F. S.-
dc.contributor.authorFraceto, Leonardo F.-
dc.subjectPeriodontitept_BR
dc.subject.otherlanguagePeriodontitispt_BR
dc.description.abstractThe 15-deoxy-(Delta 12,14)-PG J(2) (15d-PGJ(2)) has demonstrated excellent anti-inflammatory results in different experimental models. It can be used with a polymeric nanostructure system for modified drug release, which can change the therapeutic properties of the active principle, leading to increased stability and slower/prolonged release. The aim of the current study was to test a nano-technological formulation as a carrier for 15d-PGJ(2), and to investigate the immunomodulatory effects of this formulation in a mouse periodontitis model. Poly (D, L-lactide-coglycolide) nanocapsules (NC) were used to encapsulate 15d-PGJ(2). BALB/c mice were infected on days 0, 2, and 4 with Aggregatibacter actinomycetemcomitans and divided into groups (n = 5) that were treated daily during 15 d with 1, 3, or 10 mu g/kg 15d-PGJ(2)-NC. The animals were sacrificed, the submandibular lymph nodes were removed for FACS analysis, and the jaws were analyzed for bone resorption by morphometry. Immunoinflammatory markers in the gingival tissue were analyzed by reverse transcriptase-quantitative PCR, Western blotting, or ELISA. Infected animals treated with the 15d-PGJ(2)-NC presented lower bone resorption than infected animals without treatment (p < 0.05). Furthermore, infected animals treated with 10 mu g/kg 15d-PGJ(2)-NC had a reduction of CD4(+)CD25(+)FOXP3(+) cells and CD4/CD8 ratio in the submandibular lymph node (p < 0.05). Moreover, CD55 was upregulated, whereas RANKL was downregulated in the gingival tissue of the 10 mu g/kg treated group (p < 0.05). Several proinflammatory cytokines were decreased in the group treated with 10 mu g/kg 15d-PGJ(2)-NC, and high amounts of 15d-PGJ(2) were observed in the gingiva. In conclusion, the 15d-PGJ(2)-NC formulation presented immunomodulatory effects, decreasing bone resorption and inflammatory responses in a periodontitis mouse modelpt_BR
dc.relation.ispartofJournal of immunologypt_BR
dc.relation.ispartofabbreviationJ. immunol.pt_BR
dc.publisher.cityBaltimore, MDpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherAmerican Association of Immunologistspt_BR
dc.date.issued2012-
dc.date.monthofcirculationJulypt_BR
dc.language.isoengpt_BR
dc.description.volume189pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage1043pt_BR
dc.description.lastpage1052pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0022-1767pt_BR
dc.identifier.eissn1550-6606pt_BR
dc.identifier.doi10.4049/jimmunol.1200730pt_BR
dc.identifier.urlhttps://www.jimmunol.org/content/189/2/1043pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber471305/2009-0pt_BR
dc.description.sponsordocumentnumber2010/15014-9pt_BR
dc.date.available2020-09-01T16:10:26Z-
dc.date.accessioned2020-09-01T16:10:26Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-09-01T16:10:26Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:51Z : No. of bitstreams: 1 000306119800062.pdf: 660911 bytes, checksum: 65e69d58631e38b8e952e66746b7004c (MD5)en
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dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348459-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000306119800062pt_BR
dc.creator.orcid0000-0003-0765-4421pt_BR
dc.creator.orcid0000-0002-2827-2038pt_BR
dc.type.formArtigopt_BR
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