Please use this identifier to cite or link to this item:
Type: Artigo
Title: Cavities determine the pressure unfolding of proteins
Author: Roche, Julien
Caro, Jose A.
Norberto, Douglas R.
Barthe, Philippe
Roumestand, Christian
Schlessman, Jamie L.
Garcia, Angel E.
Garcia-Moreno E, Bertrand
Royer, Catherine A.
Abstract: It has been known for nearly 100 years that pressure unfolds proteins, yet the physical basis of this effect is not understood. Unfolding by pressure implies that the molar volume of the unfolded state of a protein is smaller than that of the folded state. This decrease in volume has been proposed to arise from differences between the density of bulk water and water associated with the protein, from pressure-dependent changes in the structure of bulk water, from the loss of internal cavities in the folded states of proteins, or from some combination of these three factors. Here, using 10 cavity-containing variants of staphylococcal nuclease, we demonstrate that pressure unfolds proteins primarily as a result of cavities that are present in the folded state and absent in the unfolded one. High-pressure NMR spectroscopy and simulations constrained by the NMR data were used to describe structural and energetic details of the folding landscape of staphylococcal nuclease that are usually inaccessible with existing experimental approaches using harsher denaturants. Besides solving a 100-year-old conundrum concerning the detailed structural origins of pressure unfolding of proteins, these studies illustrate the promise of pressure perturbation as a unique tool for examining the roles of packing, conformational fluctuations, and water penetration as determinants of solution properties of proteins, and for detecting folding intermediates and other structural details of protein-folding landscapes that are invisible to standard experimental approaches
Subject: Cavidades
Country: Estados Unidos
Editor: National Academy of Sciences
Rights: Fechado
Identifier DOI: 10.1073/pnas.1200915109
Date Issue: 2012
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000303602100042.pdf2.08 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.