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Type: Artigo
Title: Serum-starved apoptotic fibroblasts reduce blastocyst production but enable development to term after SCNT in cattle
Author: Miranda, Moysés dos Santos
Bressan, Fabiana Fernandes
Zecchin, Karina Gottardello
Vercesi, Anibal Eugênio
Mesquita, Ligia Garcia
Merighe, Giovana Krempel Fonseca
King, William Allan
Ohashi, Otávio Mitio
Pimentel, José Rodrigo Valim
Perecin, Felipe
Meirelles, Flávio Vieira
Abstract: Cell cycle synchronization by serum starvation (SS) induces apoptosis in somatic cells. This side effect of SS is hypothesized to negatively affect the outcome of somatic cell nuclear transfer (SCNT). We determined whether apoptotic fibroblasts affect SCNT yields. Serum-starved, adult, bovine fibroblasts were stained with annexin V-FITC/propidium iodide to allow apoptosis detection by flow cytometry. Positive and negative cells sorted by fluorescence activated cell sorting (FACS) and an unsorted control group were used as nuclear donors for SCNT. Reconstructed embryos were cultured in vitro and transferred to synchronized recipients. Apoptosis had no effect on fusion and cleavage rates; however, it resulted in reductions in blastocyst production and quality measured by apoptotic index. However, reconstructed embryos with apoptotic cells resulted in pregnancy rates similar to that of the control on day 30, and generated one live female calf. In conclusion, we showed that apoptotic cells present in serum-starved cultures negatively affect embryo production after SCNT without compromising full-term development. Further studies will evaluate the ability of the oocyte to reprogram cells in specific phases of apoptosis
Subject: Blastocisto
Country: Estados Unidos
Editor: Mary Ann Liebert
Rights: Fechado
Identifier DOI: 10.1089/clo.2009.0028
Date Issue: 2009
Appears in Collections:FCM - Artigos e Outros Documentos

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