Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348281
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampVieira-Damiani, Gislaine || Silva, Marilene Neves da || Soares, Tânia Cristina Benetti || Almeida, Amanda Roberta de || César, Carlos Lenz || Cintra, Maria Letícia || Velho, Paulo Eduardo Neves Ferreirapt_BR
dc.typeArtigopt_BR
dc.titleBartonella henselae initial infection of mature human erythrocytes observed in real time using bacterial endogenous fluorescencept_BR
dc.contributor.authorVieira-Damiani, G.pt_BR
dc.contributor.authorEricson, M. E.pt_BR
dc.contributor.authorSilva, M. N. dapt_BR
dc.contributor.authorGupta, K.pt_BR
dc.contributor.authorSoares, T. B.pt_BR
dc.contributor.authorAlmeida, A. R. dept_BR
dc.contributor.authorPelegati, V. B.pt_BR
dc.contributor.authorBaratti, M. O.pt_BR
dc.contributor.authorCesar, C. L.pt_BR
dc.contributor.authorCintra, M. L.pt_BR
dc.contributor.authorVelho, P. E. N. F.pt_BR
dc.subjectInfecções por Bartonellapt_BR
dc.subjectAnemiapt_BR
dc.subjectBartonella infectionspt_BR
dc.subjectAnemiapt_BR
dc.description.abstractBartonella henselae is a causative agent of anemia, cat scratch disease, bacillary angiomatosis, recurrent fever, hepatitis, endocarditis, chronic lymphadenopathy, joint and neurological disorders. B. henselae are intra-erythrocytic bacteria. The goal of this study was to visualize the B. henselae invasion into enucleated human red blood cells in real time using bacterium endogenous fluorescence. We took advantage of the unique fluorescence emission spectral profile of the bacteria. We used a linear unmixing approach to separate the fluorescence emission spectra of human erythrocytes from native B. henselae when excited at 488nm. Human blood samples were inoculated with B. henselae and incubated for 60 hours. 3-D live images were captured at select intervals using multi-photon laser scanning microscopy. Uninfected blood samples were also analyzed. This study revealed bacteria entering mature erythrocytes over a 60 hour time period.pt_BR
dc.relation.ispartofJournal of tropical diseases & public healthpt_BR
dc.relation.ispartofabbreviationJ. trop. Dis. public healthpt_BR
dc.publisher.cityBrusselspt_BR
dc.publisher.countryBélgicapt_BR
dc.publisherLongdom Grouppt_BR
dc.date.issued2016pt_BR
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume4pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage1pt_BR
dc.description.lastpage4pt_BR
dc.rightsabertopt_BR
dc.sourcePUBMEDpt_BR
dc.identifier.issn2329-891Xpt_BR
dc.identifier.eissn2329-891Xpt_BR
dc.identifier.doi10.4172/2329-891X.1000207pt_BR
dc.identifier.urlhttps://www.longdom.org/abstract/bartonella-henselae-initial-infection-of-mature-human-erythrocytes-observed-in-real-time-using-bacterial-endogenous-fluo-40525.htmlpt_BR
dc.description.sponsorshipSem informaçãopt_BR
dc.description.sponsorship1Sem informaçãopt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.date.available2020-08-28T19:29:31Z-
dc.date.accessioned2020-08-28T19:29:31Z-
dc.description.provenanceMade available in DSpace on 2020-08-28T19:29:31Z (GMT). No. of bitstreams: 1 5639914.pdf: 832552 bytes, checksum: c356607eca8ccf2e73a6ea698457e867 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348281-
dc.contributor.departmentDepartamento de Clínica Médica || Departamento de Clínica Médica || Departamento de Clínica Médica || Departamento de Clínica Médica || Departamento de Eletrônica Quântica || Departamento de Anatomia Patológica || Departamento de Clínica Médicapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicas || Faculdade de Ciências Médicas || Faculdade de Ciências Médicas || Faculdade de Ciências Médicas || Instituto de Física Gleb Wataghin || Faculdade de Ciências Médicas || Faculdade de Ciências Médicaspt_BR
dc.identifier.source5639914pt_BR
dc.creator.orcid0000-0001-8036-6616 || 0000-0001-6626-5632 || Sem informação || Sem informação || 0000-0001-5573-9636 || 0000-0001-7125-2238 || 0000-0001-7901-2351pt_BR
dc.type.formArtigopt_BR
dc.identifier.articleid207pt_BR
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