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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAltemani, Albina Messias de Almeida Milani-
dc.typeArtigopt_BR
dc.titleIn vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned mediumpt_BR
dc.contributor.authorMartinez, Elizabeth F.-
dc.contributor.authorDemasi, Ana Paula Dias-
dc.contributor.authorNapimoga, Marcelo Henrique-
dc.contributor.authorArana-Chavez, Victor Elias-
dc.contributor.authorAltemani, Albina-
dc.contributor.authorAraújo, Ney Soares de-
dc.contributor.authorAraújo, Vera Cavalcanti de-
dc.subjectMatriz extracelularpt_BR
dc.subject.otherlanguageExtracellular matrixpt_BR
dc.description.abstractIn order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of α-smooth muscle actin (α-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that α-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and α-SMA expression by these cells, especially in the fibronectin substratumpt_BR
dc.relation.ispartofOral oncologypt_BR
dc.relation.ispartofabbreviationOral oncol.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherElsevierpt_BR
dc.date.issued2012-
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume48pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage102pt_BR
dc.description.lastpage109pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1368-8375pt_BR
dc.identifier.eissn1879-0593pt_BR
dc.identifier.doi10.1016/j.oraloncology.2011.09.008pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1368837511008232pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2008/58722-3; 2008/58721-7pt_BR
dc.date.available2020-08-28T13:01:18Z-
dc.date.accessioned2020-08-28T13:01:18Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-08-28T13:01:18Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:29Z : No. of bitstreams: 1 000299954900007.pdf: 3401053 bytes, checksum: 1101b0f3d2d1b676dbe246cd40c84ce6 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-08-28T13:01:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348206-
dc.contributor.departmentDepartamento de Anatomia Patológicapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordMyoepithelial cellspt_BR
dc.subject.keywordPleomorphic adenomapt_BR
dc.subject.keywordFGF-2α-SMApt_BR
dc.identifier.source000299954900007pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigopt_BR
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