Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348139
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampRodrigues, Flávia Sammartino Mariano-
dc.contributor.authorunicampNociti Junior, Francisco Humberto-
dc.contributor.authorunicampMattos-Graner, Renata de Oliveira-
dc.typeArtigopt_BR
dc.titleAntimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjectspt_BR
dc.contributor.authorMariano, F.S.-
dc.contributor.authorCampanelli, A.P.-
dc.contributor.authorNociti Jr., F.H.-
dc.contributor.authorMattos-Graner, R.O.-
dc.contributor.authorGonçalves, R.B.-
dc.subjectÓxido nítricopt_BR
dc.subjectNeutrófilospt_BR
dc.subjectPeriodontitept_BR
dc.subject.otherlanguageNitric oxidept_BR
dc.subject.otherlanguageNeutrophilspt_BR
dc.subject.otherlanguagePeriodontitispt_BR
dc.description.abstractNeutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS (Ec-LPS). qRT-PCR was used to determine quantities of HNP 1-3 and LL-37 mRNA in neutrophils. Amounts of HNP 1-3 and LL-37 proteins in the cell culture supernatants were also determined by ELISA. In addition, NO levels in neutrophil culture supernatants were quantitated by the Griess reaction. Neutrophils from periodontitis patients cultured with Aa-LPS, Pg-LPS and Ec-LPS expressed higher HNP 1-3 mRNA than neutrophils from healthy subjects. LL-37 mRNA expression was higher in neutrophils from patients stimulated with Aa-LPS. Neutrophils from periodontitis patients produced significantly higher LL-37 protein levels than neutrophils from healthy subjects when stimulated with Pg-LPS and Ec-LPS, but no difference was observed in HNP 1-3 production. Neutrophils from periodontitis patients cultured or not with Pg-LPS and Ec-LPS produced significantly lower NO levels than neutrophils from healthy subjects. The significant differences in the production of LL-37 and NO between neutrophils from healthy and periodontitis subjects indicate that production of these molecules might influence individual susceptibility to important periodontal pathogens.pt_BR
dc.relation.ispartofBrazilian journal of medical and biological researchpt_BR
dc.relation.ispartofabbreviationBraz. j. med. biol. res.pt_BR
dc.publisher.cityRibeirão Preto, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherAssociação Brasileira de Divulgação Científicapt_BR
dc.date.issued2012-
dc.date.monthofcirculationNov.pt_BR
dc.language.isoengpt_BR
dc.description.volume45pt_BR
dc.description.issuenumber11pt_BR
dc.description.firstpage1017pt_BR
dc.description.lastpage1024pt_BR
dc.rightsAbertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.eissn1414-431Xpt_BR
dc.identifier.doi10.1590/S0100-879X2012007500123pt_BR
dc.identifier.urlhttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100004pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2007/00219-1pt_BR
dc.date.available2020-08-27T16:36:01Z-
dc.date.accessioned2020-08-27T16:36:01Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2020-08-27T16:36:01Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:24Z : No. of bitstreams: 1 S0100-879X2012001100004.pdf: 493501 bytes, checksum: 3a9143dddb2027d23ef028cc3b2fd246 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-08-27T16:36:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348139-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.departmentDepartamento de Diagnóstico Oralpt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordAntimicrobial peptidespt_BR
dc.subject.keywordInnate immunitypt_BR
dc.identifier.sourceS0100-879X2012001100004pt_BR
dc.creator.orcid0000-0002-4542-4400pt_BR
dc.creator.orcid0000-0001-6809-8866pt_BR
dc.creator.orcid0000-0001-8309-8135pt_BR
dc.type.formArtigopt_BR
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