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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampOliveira-Maekawa, Vanessa de-
dc.contributor.authorunicampSemir, João-
dc.contributor.authorunicampForni-Martins, Eliana Regina-
dc.typeArtigopt_BR
dc.titleKaryotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniquespt_BR
dc.contributor.authorOliveira, V. M.-
dc.contributor.authorMansanares, M. E.-
dc.contributor.authorSemir, J.-
dc.contributor.authorForni-Martins, E. R.-
dc.subjectVernoniapt_BR
dc.subject.otherlanguageVernoniapt_BR
dc.description.abstractIn order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA3+ terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA+ and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes. In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA3+ terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA+ and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomespt_BR
dc.relation.ispartofGenetics and molecular researchpt_BR
dc.relation.ispartofabbreviationGenet. mol. res.pt_BR
dc.publisher.cityRibeirão Preto, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherFUNPECRPpt_BR
dc.date.issued2012-
dc.date.monthofcirculationDec.pt_BR
dc.language.isoengpt_BR
dc.description.volume11pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage4204pt_BR
dc.description.lastpage4212pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1676-5680pt_BR
dc.identifier.doi10.4238/2012.September.25.1pt_BR
dc.identifier.urlhttps://www.geneticsmr.com/articles/1879pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumber04/13165-9pt_BR
dc.date.available2020-08-27T11:57:46Z-
dc.date.accessioned2020-08-27T11:57:46Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-08-27T11:57:46Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:18Z : No. of bitstreams: 1 000313960500065.pdf: 455661 bytes, checksum: 00acb69c03bfc0f5dabfcce9c4ebe9e0 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-08-27T11:57:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348088-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Botânicapt_BR
dc.contributor.departmentDepartamento de Biologia Vegetalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordAgNOR bandingpt_BR
dc.subject.keywordCMA bandingpt_BR
dc.subject.keywordPhysical mappingpt_BR
dc.subject.keyword45S rDNA sitept_BR
dc.identifier.source000313960500065pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-8889-4955pt_BR
dc.type.formArtigo de pesquisapt_BR
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