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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampPereira, Aline Ferreira de Oliveira-
dc.typeArtigopt_BR
dc.titleOral immunization with attenuated Salmonella vaccine expressing Escherichia coli O157:H7 intimin gamma triggers both systemic and mucosal humoral immunity in micept_BR
dc.contributor.authorOliveira, Aline Ferreira-
dc.contributor.authorCardoso, Silvia Almeida-
dc.contributor.authordos Reis Almeida, Fausto Bruno-
dc.contributor.authorde Oliveira, Leandro Licursi-
dc.contributor.authorPitondo-Silva, Andre-
dc.contributor.authorSoares, Sandro Gomes-
dc.contributor.authorHanna, Ebert Seixas-
dc.subjectEscherichia colipt_BR
dc.subject.otherlanguageEscherichia colipt_BR
dc.description.abstractHuman infections with EHEC such as O157:H7 have been a great concern for worldwide food-industry surveillance. This pathogen is commonly associated with bloody diarrhea that can evolve to the life-threatening hemolytic uremic syndrome. Animals are the natural reservoir where this pathogen remains asymptomatically, in steps of ingestion and colonization of the bowel. The bacterium is shed in the feces, contaminating the surroundings, including water and food that are directed for human consumption. A major player in this colonization process is intimin, an outer membrane adhesion molecule encoded by the E. coli attachment and effacement (eae) gene that has been shown to be essential for intimate bacterial attachment to eukaryotic host cells. In an attempt to reduce the colonization of animal reservoirs with EHEC O157:H7, we designed a vaccine model to induce an immune response against intimin gamma. The model is based on its recombinant expression in attenuated Salmonella, used as a suitable vaccine vector because of its recognized ability to deliver recombinant antigens and to elicit all forms of immunity: mucosal, systemic, and humoral responses. To test this model, mice were orally immunized with a S. enterica serovar Typhimurium strain carrying the pYA3137eaeA vector, and challenged with E. coli O157:H7. Here we show that immunization induced the production of high levels of specific IgG and IgA antibodies and promoted reduction in the fecal shedding of EHEC after challenge. The live recombinant vaccine reported herein may contribute to the efforts of reducing animal intestinal mucosa colonizationpt_BR
dc.relation.ispartofMicrobiology and immunologypt_BR
dc.relation.ispartofabbreviationMicrobiol immunolpt_BR
dc.publisher.cityRichmondpt_BR
dc.publisher.countryAustraliapt_BR
dc.publisherWileypt_BR
dc.date.issued2012-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume56pt_BR
dc.description.issuenumber8pt_BR
dc.description.firstpage513pt_BR
dc.description.lastpage522pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0385-5600pt_BR
dc.identifier.eissn1348-0421pt_BR
dc.identifier.doi10.1111/j.1348-0421.2012.00477.xpt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1348-0421.2012.00477.xpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber573232/2008‐3; 476494/2009‐5; 350280/2008‐8pt_BR
dc.description.sponsordocumentnumber03/00501‐8; 06/60941‐0; 05/04465‐1; 06/50694‐5; 08/57354‐0; 08/50602‐9pt_BR
dc.date.available2020-08-26T12:46:13Z-
dc.date.accessioned2020-08-26T12:46:13Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-08-26T12:46:13Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:14:12Z : No. of bitstreams: 1 000306896900003.pdf: 399240 bytes, checksum: a11f3526a99eb04e4b90ddd97c5d5e05 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-08-26T12:46:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348005-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordSalmonella entericapt_BR
dc.subject.keywordTyphimuriumpt_BR
dc.subject.keywordVaccinespt_BR
dc.identifier.source000306896900003pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.type.formArtigo originalpt_BR
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