Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/347532
Type: | Artigo |
Title: | Genetic diversity in cultivated carioca common beans based on molecular marker analysis |
Author: | Perseguini, Juliana Morini Küpper Cardoso Chioratto, Alisson Fernando Zucchi, Maria Imaculada Colombo, Carlos Augusto Carbonell, Sérgio Augusto Moraes Mondego, Jorge Mauricio Costa Gazaffi, Rodrigo Garcia, Antonio Augusto Franco Campos, Tatiana de Souza, Anete Pereira de Rubiano, Luciana Benchimol |
Abstract: | A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm |
Subject: | Estrutura genética Feijão |
Country: | Genet mol. biol |
Editor: | Sociedade Brasileira de Genética |
Rights: | Aberto |
Identifier DOI: | 10.1590/S1415-47572011000100017 |
Address: | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
Date Issue: | 2011 |
Appears in Collections: | IB - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
S1415-47572011000100017.pdf | 464.55 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.