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Type: Artigo
Title: The Arabidopsis bZIP Gene AtbZIP63 Is a sensitive integrator of transient abscisic acid and glucose signals
Author: Matiolli, Cleverson Carlos
Tomaz, Juarez Pires
Duarte, Gustavo Turqueto
Prado, Fernanda Manso
Del Bem, Luiz Eduardo Vieira
Silveira, Amanda Bortolini
Gauer, Luciane
Corrêa, Luiz Gustavo Guedes
Drumond, Rodrigo Duarte
Viana, Amárico Josá Carvalho
Di Mascio, Paolo
Meyer, Christian
Vincentz, Michel
Abstract: Glucose modulates plant metabolism, growth, and development. In Arabidopsis (Arabidopsis thaliana), Hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. Here, we show that the intensity of short-term responses to glucose can vary with ABA activity. We report that the transient (2 h/4 h) repression by 2% glucose of AtbZIP63, a gene encoding a basic-leucine zipper (bZIP) transcription factor partially involved in the Snf1-related kinase KIN10-induced responses to energy limitation, is independent of HXK1 and is not mediated by changes in ABA levels. However, high-concentration (6%) glucose-mediated repression appears to be modulated by ABA, since full repression of AtbZIP63 requires a functional ABA biosynthetic pathway. Furthermore, the combination of glucose and ABA was able to trigger a synergistic repression of AtbZIP63 and its homologue AtbZIP3, revealing a shared regulatory feature consisting of the modulation of glucose sensitivity by ABA. The synergistic regulation of AtbZIP63 was not reproduced by an AtbZIP63 promoter-5′-untranslated region::β-glucuronidase fusion, thus suggesting possible posttranscriptional control. A transcriptional inhibition assay with cordycepin provided further evidence for the regulation of mRNA decay in response to glucose plus ABA. Overall, these results indicate that AtbZIP63 is an important node of the glucose-ABA interaction network. The mechanisms by which AtbZIP63 may participate in the fine-tuning of ABA-mediated abiotic stress responses according to sugar availability (i.e., energy status) are discussed.
Subject: Glicose
Fatores de transcrição de zíper de leucina básica
Country: Estados Unidos
Editor: American Society of Plant Biologists
Rights: Fechado
Identifier DOI: 10.1104/pp.111.181743
Date Issue: 2011
Appears in Collections:IB - Artigos e Outros Documentos

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