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Type: Artigo
Title: Dissemination of enterococcus faecalis and enterococcus faecium in a ricotta processing plant and evaluation of pathogenic and antibiotic resistance profiles
Author: Fernandes, Meg da Silva
Fujimoto, Graciela
Souza, Leandro Pio de
Kabuki, Dirce Yorika
Silva, Márcio José da
Kuaye, Arnaldo Yoshiteru
Abstract: In this work, the sources of contamination by Enterococcus spp. in a ricotta processing line were evaluated. The isolated strains were tested for virulence genes (gelE, cylA,B, M, esp, agg, ace, efaA, vanB), expression of virulence factors (hemolysin and gelatinase), and the resistance to 10 different antibiotics. Enterococcus faecium and Enterococcus faecalis were subjected to discriminatory identification by intergenic spacer region (ITS)‐polymerase chain reaction and sequencing of the ITS region. The results showed that Enterococcus spp. was detected in the raw materials, environment samples and the final product. None of the 107 Enterococcus isolates were completely free from all virulence genes considered. A fraction of 21.5% of the isolates containing all of the genes of the cylA, B, M operon also expressed β‐hemolysis. Most of the isolates showed the gelE gene, but only 9.3% were able to hydrolyze gelatin. In addition, 23.5% of the observed Enterococcus isolates had the vanB gene but were susceptible to vancomycin in vitro. The dissemination of antibiotic‐resistant enterococci was revealed in this study: 19.3% of the E. faecium samples and 78.0% of the E. faecalis samples were resistant to at least one of the antibiotics tested. Sequencing of region discriminated 5 and 7 distinct groups among E. faecalis and E. faecium, respectively. Although some similarity was observed among some of the isolates, all E. faecalis and E. faecium isolates had genetic differences both in the ITS region and in the virulence profile, which makes them different from each other
Subject: Ricota
Country: Estados Unidos
Editor: Wiley
Rights: Fechado
Identifier DOI: 10.1111/1750-3841.12824
Date Issue: 2015
Appears in Collections:FEA - Artigos e Outros Documentos
CBMEG - Artigos e Outros Documentos

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