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Type: Artigo
Title: Tocopherols and tocotrienols: an adapted methodology by UHPLC/MS without sample pretreatment steps
Author: Ansolin, Marina
Souza, Patricia Tonon de
Meirelles, Antônio José de Almeida
Batista, Eduardo Augusto Caldas
Abstract: Ultra high-performance liquid chromatography-electrospray ionization-mass spectrometry (UHPLC/ESI/MS) methodology was adapted for identification and quantification of tocopherols and tocotrienols in vegetable oils with no derivatization or sample preparation steps. The UHPLC analysis was performed using a C18 column and mobile phase composed of methanol: water: ammonium hydroxide (99:1:0.1 v/v/v) and isopropanol. A single mass spectrometer with electrospray on negative mode was used as a detector for tocopherols and tocotrienols. The samples were diluted in isopropanol. The limit of quantification for tocopherols was 0.006 μg mL−1, and the linear range was 0.006 to 0.01 μg mL−1; for tocotrienols, the limit of quantification was 0.002 μg mL−1, and the linear range of analysis was 0.002 to 0.003 μg mL−1. The correlation coefficients were higher than 0.99, indicating that the method has suitable linearity. The methodology has proven to be precise, reproducible, and robust for the parameters studied
Subject: Espectrometria de massa
Country: Estados Unidos
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s12161-016-0768-z
Date Issue: 2017
Appears in Collections:IQ - Artigos e Outros Documentos
FEA - Artigos e Outros Documentos

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