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Type: Artigo
Title: The structuring of high molecular weight hyaluronic acid in microparticles or sponges improves its performance when associated with platelet-rich plasma
Author: Shimojo, Andréa Arruda Martins
Galdames, Sofia Elisa Moraga
Duarte, Adriana da Silva Santos
Pina, Lucas Martins
Rodrigues, Ana Amélia
Luzo, Ângela Cristina Malheiros
Belangero, William Dias
Santana, Maria Helena Andrade
Abstract: High molecular weight hyaluronic acid (HA) has been widely used with platelet-rich plasma (PRP) in regenerative medicine. This study aimed to investigate the in vitro association between HA crosslinked with 1,4-butanediol diglycidyl ether (BDDE), structured in microparticles and sponges, and PRP as composite scaffolds for growth factor release, proliferation of human adipose-derived mesenchymal stem cells and differentiation by the osteogenic marker alkaline phosphatase. The reason for using BDDE is to increase the residence time in vivo, improve the mechanical stability of free HA (fHA) and provide a larger surface area for cell proliferation and differentiation. PRP was obtained via controlled centrifugation of whole blood and activated with autologous serum and calcium. The results showed that structuration benefited the physicochemical and mechanical properties compared to fHA. Both structures were non-cytotoxic. The release of growth factors from PRP was higher than fHA but similar to fibrin network. The effects on the proliferation of seeded mesenchymal cells were not intense. However, a remarkable activity of the osteogenic marker alkaline phosphatase was observed. We concluded that the structuration of HA provides injectable or solid formulations with better properties compared to fHA, which associated with PRP may be promising for regeneration of bone tissue
Subject: Ácido hialurônico
Country: Índia
Editor: Society for Biomaterials and Artificial Organs
Rights: Fechado
Date Issue: 2015
Appears in Collections:FCM - Artigos e Outros Documentos
FEQ - Artigos e Outros Documentos
HEMO - Artigos e Outros Documentos

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