Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/346390
Type: Artigo
Title: Performance of PRP associated with porous chitosan as a composite scaffold for regenerative medicine
Author: Shimojo, Andréa Arruda Martins
Perez, Amanda Gomes Marcelino
Galdames, Sofia Elisa Moraga
Brissac, Isabela Cambraia de Souza
Santana, Maria Helena Andrade
Abstract: This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days
Subject: Quitosana
Country: Estados Unidos
Editor: Hindawi
Rights: Aberto
Identifier DOI: 10.1155/2015/396131
Address: https://www.hindawi.com/journals/tswj/2015/396131/
Date Issue: 2015
Appears in Collections:FEQ - Artigos e Outros Documentos

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