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Type: Artigo
Title: Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine
Author: Melo, Bruna Alice Gomes de
França, Carla Giometti
Dávila, José Luis
Batista, Nilza Alzira
d'Ávila, Marcos Akira
Luzo, Ângela Cristina Malheiros
Lana, José Fabio Santos Duarte
Santana, Maria Helena Andrade
Abstract: Autologous leukocyte- and platelet-rich plasma (L-PRP) combined with hyaluronic acid (HA) has been widely used in local applications for cartilage and bone regeneration. The association between L-PRP and HA confers structural and rheological changes that differ among individual biomaterials but has not been investigated. Therefore, the standardization and characterization of L-PRP-HA are important to consider when comparing performance results to improve future clinical applications. To this end, we prepared semi-interpenetrating polymer networks (semi-IPNs) of L-PRP and HA and characterized their polymerization kinetics, morphology, swelling ratio, stability and rheological behavior, which we found to be tunable according to the HA molar mass (MM). Mesenchymal stem cells derived from human adipose tissue (h-AdMSCs) seeded in the semi-IPNs had superior viability and chondrogenesis and osteogenesis capabilities compared to the viability and capabilities of fibrin. We have demonstrated that the preparation of the semi-IPNs under controlled mixing ensured the formation of cell-friendly hydrogels rich in soluble factors and with tunable properties according to the HA MM, rendering them suitable for clinical applications in regenerative medicine
Subject: Ácido hialurônico
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.msec.2019.110547
Date Issue: 2020
Appears in Collections:FCM - Artigos e Outros Documentos
FEQ - Artigos e Outros Documentos
HEMO - Artigos e Outros Documentos
FEM - Artigos e Outros Documentos

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