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|Title:||Separation of verapamil enantiomers by high performance liquid chromatography using amylose-based stationary phase|
|Author:||Perna, Rafael F.|
Cremasco , Marco A.
Santana, Cesar C.
|Abstract:||Verapamil is a drug used in the therapy of hypertension, supraventricular arrhythmias and angina pectoris, was separate by high performance liquid chromatography (HPLC) using a semipreparative column containing amylose tris (3,5-dimethylphenyl) carbamate as chiral stationary phase. The effects of temperature (293–308 K), flow rate (1.0–2.5 mL min-1 ) and polar modifiers 2- propanol and ethanol ratio (2.5 % - 7.5 %) (by volume) on separation parameters chromatographic were evaluated. The experiments were carried out under normal phase conditions, and the chiral separation exhibit satisfactory values of capacity factors, selectivity and resolution for verapamil enantiomers with a good performance. The preferred operation conditions were 298 K, 1.0 mL min1 , and the 2-propanol and ethanol ratio in the mobile phase 5.0 % (by volume)|
|Appears in Collections:||FEQ - Artigos e Outros Documentos|
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