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|Title:||Association between cationic liposomes and low molecular weight hyaluronic acid|
|Author:||Gasperini, Antonio A. M.|
Puentes-Martinez, Ximena E.
Balbino, Tiago Albertini
Rigoletto, Thais de Paula
Corrêa, Gabriela de Sá Cavalcanti
Portugal, Rodrigo Villares
Torre, Lucimara Gaziola de La
Cavalcanti, Leide P.
|Abstract:||This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential|
|Editor:||American Chemical Society|
|Appears in Collections:||FEQ - Artigos e Outros Documentos|
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