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Type: Artigo
Title: Fatty acid is a potential agent for bone tissue induction : In vitro and in vivo approach
Author: Cardoso, Guinea BC
Chacon, Erivelto
Chacon, Priscila GL
Bordeaux-Rego, Pedro
Duarte, Adriana SS
Saad, Sara T Olalla
Zavaglia, Cecilia AC
Cunha, Marcelo R
Abstract: Our hypothesis was to investigate the fatty acid potential as a bone induction factor. In vitro and in vivo studies were performed to evaluate this approach. Oleic acid was used in a 0.5 wt.% concentration. Polycaprolactone was used as the polymeric matrix by combining solvent-casting and particulate-leaching techniques, with a final porosity of 70 wt.%, investigated by SEM images. Contact angle measurements were produced to investigate the influence of oleic acid on polycaprolactone chains. Cell culture was performed using adipocyte-derived stem cells to evaluate biocompatibility and bioactivity properties. In addition, in vivo studies were performed to evaluate the induction potential of oleic acid addition. Adipocyte-derived stem cells were used to provide differentiation after 21 days of culture. Likewise, information were obtained with in vivo data and cellular invagination was observed on both scaffolds (polycaprolactone and polycaprolactone /oleic acid); interestingly, the scaffold with oleic acid addition demonstrated that cellular migrations are not related to the surrounding tissue, indicating bioactive potential. Our hypothesis is that fatty acid may be used as a potential induction factor for bone tissue engineering. The study’s findings indicate oleic acid as a possible agent for bone induction, according to data on cell differentiation, proliferation, and migration
Subject: Ácidos graxos
Country: Estados Unidos
Editor: Society for Experimental Biology and Medicine
Rights: Aberto
Identifier DOI: 10.1177/1535370217731104
Date Issue: 2017
Appears in Collections:FCM - Artigos e Outros Documentos
FEM - Artigos e Outros Documentos

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