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|Title:||Comparative research of bacteria gram-negative and positive on ti-30ta alloy|
|Abstract:||The aim of this study was comparative research of response to bacteria Gram-negative and Gram-positive on Ti-30Ta alloy and Ti cp for dental implant application. Nowadays, the most used way to replace teeth lost is a dental implant. The common material used in dentistry is Titanium due to the high strength, durability, and biocompatibility. However, the elastic modulus of Ti Cp is 105 GPa and the bone around 15-25 GPa its results in a stress shielding problem. Titanium with 30% tantalum results to alloy with an elastic modulus at 45 GPa. It is near to the spot bone. Studies have been shown the most failure of implantation is caused by infections. Staphylococcus aureus (S. aureus) and Escherichia coli are the most frequent bacterial strains found on the sources of infections. Thus, we investigated two gram-positive (S aureus and S. epidermidis) and gram-negative bacteria (Escherichia coli and P. aeruginosa) on Ti cp and Ti-30Ta alloy surface. So, the ideal material to dental implant must have properties to prevent and reduce bacterial-associated implant failure. The Ti-30Ta alloy was fabricated by mixing Ti cp and Tantalum. S. aureus, S. epidermidis, Escherichia coli and P. aeruginosa were selected to investigate the antimicrobial activity. The number of colony-forming units per milliliter (CFU/mL) was determined and analyzed statistically (ANOVA, Tukey test, p < 0.05). Characterization techniques such as scanning electron microscopy (SEM) were used in order to investigate the surface of samples. The results demonstrate the influence of Ti-30Ta alloy surface on bacterial adhesion on gram-positive strains. Significant statistical differences for bacterial adhesion of S epidermidis (p < 0.05) and S aureus (p < 0,001) were observed Ti-30Ta surface compared to Ti cp [Figure 1]. SEM images show Ti-30Ta alloy surface after the biofilms have been grown by incubated at 37 °C for 48 h. The surface of Ti-30Ta decreases bacterial adhesion of S epidermidis and S aureus. Gram-positive bacteria showed a decrease of adhesion because of the cell walls thicker and harder, absence of outer membrane and presence of proteins, lipids and techoic acid.The novel Ti-30Ta alloy has an influence on Gram-positive bacterial adhesion as you can see the number of colony-forming units per millimeter has been decreasing. Thus, Ti-30Ta alloy can be promising in the dental implant field because of the potential to prevent implant infection and the elastic modulus near to the bone|
|Appears in Collections:||FEM - Artigos e Outros Documentos|
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