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Type: Artigo
Title: Idiopathic atrophoderma of Pasini and Pierini: a case study of collagen and elastin texture by multiphoton microscopy
Author: Vieira-Damiani, G.
Lage, D.
Daldon, P. E. C.
Alves, C. R. T.
Cintra, M. L.
Metze, K.
Adur, J.
Pelegati, V. B.
Carvalho, H. F.
Cesar, C. L.
Abstract: Background: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. Objective: To identify histologic features that distinguish IAPP from unaffected skin. Methods: Eleven patients were included for conventional analyses. Masson trichromee and Unna-Tanzer orcein-stained sections were evaluated using automated morphometry. Hematoxylin-eosinestained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. Results: No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. Limitations: The low number of patients evaluated. Conclusion: The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses.
Subject: Atrofia
Fibra de colágeno
Fibras elásticas
Geração de segundo harmônico
Fluorescência excitada por dois fótons
Collagen fiber
Fibers elastic
Second harmonic generation
Two photon excited fluorescence
Country: Estados Unidos
Editor: Mosby
Rights: fechado
Identifier DOI: 10.1016/j.jaad.2017.02.044
Date Issue: 2017
Appears in Collections:IFGW - Artigos e Outros Documentos
IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos

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