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Type: Artigo
Title: Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail
Author: Bussamra, Bianca Consorti
Freitas, Sindelia
Costa, Aline Carvalho da
Abstract: The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and β-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5 L converts 49.11% ± 0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification
Subject: Glicosídeo hidrolases
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.biortech.2015.03.117
Date Issue: 2015
Appears in Collections:FEQ - Artigos e Outros Documentos

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