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Type: Artigo
Title: Bioethanol production by recycled Scheffersomyces stipitis in sequential batch fermentations with high cell density using xylose and glucose mixture
Author: Santos, Samantha Christine
Sousa, Amanda Silva de
Dionísio, Suzane Rodrigues
Tramontina, Robson
Ruller, Roberto
Squina, Fabio Márcio
Rossell, Carlos Eduardo Vaz
Costa, Aline Carvalho da
LutzIenczak, Jaciane
Abstract: Here, it is shown three-step investigative procedures aiming to improve pentose-rich fermentations performance, involving a simple system for elevated mass production by Scheffersomyces stipitis (I), cellular recycle batch fermentations (CRBFs) at high cell density using two temperature strategies (fixed at 30 °C; decreasing from 30 to 26 °C) (II), and a short-term adaptation action seeking to acclimatize the microorganism in xylose rich-media (III). Cellular propagation provided 0.52 gdry cell weight gRS−1, resulting in an expressive value of 45.9 gdry cell weight L−1. The yeast robustness in CRBF was proven by effective ethanol production, reaching high xylose consumption (81%) and EtOH productivity (1.53 g L−1 h−1). Regarding the short-term adaptation, S. stipitis strengthened its robustness, as shown by a 6-fold increase in xylose reductase (XR) activity. The short fermentation time (20 h for each batch) and the fermentation kinetics for ethanol production from xylose are quite promising
Subject: Xilose
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.biortech.2016.07.102
Date Issue: 2016
Appears in Collections:FEQ - Artigos e Outros Documentos

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