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Type: Artigo
Title: Optimization of lipids production by Cryptococcus laurentii 11 using cheese whey with molasses
Author: Castanha, Rodrigo Fernandes
Mariano, Adriano Pinto
Morais, Lilia Aparecida Salgado de
Scramin, Shirlei
Monteiro, Regina Teresa Rosim
Abstract: This study aimed the optimization of culture condition and composition for production of Cryptococcus laurentii 11 biomass and lipids in cheese whey medium supplemented with sugarcane molasses. The optimization of pH, fermentation time, and molasses concentration according to a full factorial statistical experimental design was followed by a Plackett-Burman experimental design, which was used to determine whether the supplementation of the culture medium by yeast extract and inorganic salts could provide a further enhancement of lipids production. The following conditions and composition of the culture medium were found to optimize biomass and lipids production: 360 h fermentation, 6.5 pH and supplementation of (g L-1): 50 molasses, 0.5 yeast extract, 4 KH2PO4, 1 Na2HPO4, 0.75 MgSO4•7H2O and 0.002 ZnSO4•H2O. Additional supplementation with inorganic salts and yeast extract was essential to optimize the production, in terms of product concentration and productivity, of neutral lipids by C. laurentii 11. Under this optimized condition, the production of total lipids increased by 133% in relation to control experiment (from 1.27 to 2.96 g L-1). The total lipids indicated a predominant (86%) presence of neutral lipids with high content of 16- and 18- carbon-chain saturated and monosaturated fatty acids. This class of lipids is considered especially suitable for the production of biodiesel
Subject: Biodiesel
Country: Brasil
Editor: Sociedade Brasileira de Microbiologia
Rights: Aberto
Identifier DOI: 10.1590/S1517-83822014000200003
Date Issue: 2014
Appears in Collections:FEQ - Artigos e Outros Documentos

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