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Type: Artigo
Title: Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
Author: Souza, Patrícia R M
Zárate-Bladés, Carlos R
Hori, Juliana I
Ramos, Simone G
Lima, Deison S
Schneider, Tatiana
Rosada, Rogério S
Torre, Lucimara G L
Santana, Maria Helena A
Brandão, Izaíra T
Masson, Ana P
Coelho-Castelo, Arlete A M
Bonato, Vânia L
Galetti, Fabio C S
Gonçalves, Eduardo D
Botte, Domingos A
Machado, Jeanne B M
Silva, Celio L
Abstract: Tuberculosis is a major threat to human health. The high disease burden remains unaffected and the appearance of extremely drug-resistant strains in different parts of the world argues in favor of the urgent need for a new effective vaccine. One of the promising candidates is heat-shock protein 65 when used as a genetic vaccine (DNAhsp65). Nonetheless, there are substantial data indicating that BCG, the only available anti-TB vaccine for clinical use, provides other important beneficial effects in immunized infants. Methods: We compared the protective efficacy of BCG and Hsp65 antigens in mice using different strategies: i) BCG, single dose subcutaneously; ii) naked DNAhsp65, four doses, intramuscularly; iii) liposomes containing DNAhsp65, single dose, intranasally; iv) microspheres containing DNAhsp65 or rHsp65, single dose, intramuscularly; and v) prime–boost with subcutaneous BCG and intramuscular DNAhsp65. Results: All the immunization protocols were able to protect mice against infection, with special benefits provided by DNAhsp65 in liposomes and prime–boost strategies. Conclusion: Among the immunization protocols tested, liposomes containing DNAhsp65 represent the most promising strategy for the development of a new anti-TB vaccine
Subject: Lipossomos
Country: Reino Unido
Editor: Taylor & Francis
Rights: Fechado
Identifier DOI: 10.1517/14712598.8.9.1255
Date Issue: 2008
Appears in Collections:FEQ - Artigos e Outros Documentos

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