Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/343092
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAlmeida, Amanda Bandeira de-
dc.contributor.authorunicampSantos, Elis Janaína Lira dos-
dc.contributor.authorunicampAbuna, Gabriel Flores-
dc.contributor.authorunicampSalmon, Cristiane Ribeiro-
dc.contributor.authorunicampCasati, Marcio Zaffalon-
dc.contributor.authorunicampSilverio, Karina Gonzales-
dc.contributor.authorunicampNociti Junior, Francisco Humberto-
dc.typeArtigopt_BR
dc.titleIsolation and characterization of a human cementocyte-like cell line, HCY-23pt_BR
dc.contributor.authorAlmeida, A.B.-
dc.contributor.authorSantos, E.J.L.D.-
dc.contributor.authorAbuna, G.F.-
dc.contributor.authorRibeiro, C.S.-
dc.contributor.authorCasati, M.Z.-
dc.contributor.authorRuiz, K.G.S.-
dc.contributor.authorNociti Junior, F.H.-
dc.subjectCemento dentáriopt_BR
dc.subject.otherlanguageDental cementumpt_BR
dc.description.abstractCementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regenerationpt_BR
dc.relation.ispartofBrazilian oral researchpt_BR
dc.relation.ispartofabbreviationBraz. oral res.pt_BR
dc.publisher.citySão Paulo, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherSociedade Brasileira de Pesquisa Odontologicapt_BR
dc.date.issued2019-
dc.language.isoengpt_BR
dc.description.volume33pt_BR
dc.rightsAbertopt_BR
dc.sourceSCOPUSpt_BR
dc.identifier.issn1806-8324pt_BR
dc.identifier.eissn1807-3107pt_BR
dc.identifier.doi10.1590/1807-3107bor-2019.vol33.0058pt_BR
dc.identifier.urlhttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber304680/2014-1pt_BR
dc.description.sponsordocumentnumber2015/07232-0; 2015/06372-2pt_BR
dc.date.available2020-06-10T14:44:27Z-
dc.date.accessioned2020-06-10T14:44:27Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2020-06-10T14:44:27Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-09-03T11:56:11Z : No. of bitstreams: 1 2-s2.0-85071777193.pdf: 618922 bytes, checksum: f2956e68161c07a7b473e7b0c22defbf (MD5)en
dc.description.provenanceMade available in DSpace on 2020-06-10T14:44:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/343092-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordGene Expressionpt_BR
dc.subject.keywordCell Culture Techniquespt_BR
dc.identifier.source2-s2.0-85071777193pt_BR
dc.creator.orcid0000-0003-4293-0831pt_BR
dc.creator.orcid0000-0002-0324-3863pt_BR
dc.creator.orcid0000-0002-9812-488pt_BR
dc.creator.orcid0000-0003-3304-5064pt_BR
dc.creator.orcid0000-0001-9234-0536pt_BR
dc.creator.orcid0000-0001-5879-9095pt_BR
dc.creator.orcid0000-0001-6809-8866pt_BR
dc.type.formArtigo de pesquisa originalpt_BR
dc.identifier.articleide058pt_BR
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