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Type: Artigo de periódico
Title: The post-transcriptional gene silencing pathway in Eucalyptus
Author: Sassaki, Flávio Tetsuo
Campos-Pereira, Tiago
Maia, Ivan de Godoy
Abstract: Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of similarity (E value < 10-40) to proteins involved in PTGS in plants and other organisms. We conclude that most of the genes known to be involved in the PTGS pathway are represented in the FORESTs database.
Subject: gene silencing
RNA silencing
Editor: Sociedade Brasileira de Genética
Rights: aberto
Identifier DOI: 10.1590/S1415-47572005000400003
Date Issue: 1-Jan-2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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