Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/343032
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampNascimento, Juliana Minardi-
dc.contributor.authorunicampSaia-Cereda, Verônica Aparecida Monteiro-
dc.contributor.authorunicampMartins-de-Souza, Daniel-
dc.typeArtigopt_BR
dc.titleHuman cerebral organoids and fetal brain tissue share proteomic similaritiespt_BR
dc.contributor.authorNascimento, Juliana Minardi-
dc.contributor.authorSaia-Cereda, Verônica M.-
dc.contributor.authorSartore, Rafaela C.-
dc.contributor.authorCosta, Rodrigo Madeiro da-
dc.contributor.authorSchitine, Clarissa S.-
dc.contributor.authorFreitas, Hercules Rezende-
dc.contributor.authorMurgu, Michael-
dc.contributor.authorReis, Ricardo A. de Melo-
dc.contributor.authorRehen, Stevens K.-
dc.contributor.authorMartins-de-Souza, Daniel-
dc.subjectOrganoidespt_BR
dc.subjectProteômicapt_BR
dc.subject.otherlanguageOrganoidspt_BR
dc.subject.otherlanguageProteomicspt_BR
dc.description.abstractThe limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseasespt_BR
dc.relation.ispartofFrontiers in cell and developmental biologypt_BR
dc.publisher.cityLausannept_BR
dc.publisher.countrySuiçapt_BR
dc.publisherFrontiers Research Foundationpt_BR
dc.date.issued2019-
dc.date.monthofcirculationNov.pt_BR
dc.language.isoengpt_BR
dc.description.volume7pt_BR
dc.rightsAbertopt_BR
dc.sourceSCOPUSpt_BR
dc.identifier.eissn2296-634Xpt_BR
dc.identifier.doi10.3389/fcell.2019.00303pt_BR
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fcell.2019.00303/fullpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFINANCIADORA DE ESTUDOS E PROJETOS - FINEPpt_BR
dc.description.sponsorshipFUNDAÇÃO CARLOS CHAGAS FILHO DE AMPARO À PESQUISA DO ESTADO DO RIO DE JANEIRO - FAPERJpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumber14/21035-0; 16/07332-7; 13/08711-3; 14/10068-4pt_BR
dc.date.available2020-06-09T18:49:20Z-
dc.date.accessioned2020-06-09T18:49:20Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-06-09T18:49:20Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-09-03T11:56:08Z : No. of bitstreams: 1 2-s2.0-85076909909.pdf: 9660273 bytes, checksum: 8fc4fec7c86cd492157e03ce5d9c1dc3 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-06-09T18:49:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/343032-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordProteomic Similaritiespt_BR
dc.identifier.source2-s2.0-85076909909pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-3595-5846pt_BR
dc.type.formArtigo originalpt_BR
dc.description.sponsorNoteJN, VS-C, and DM-D-S are supported by the São Paulo Research Foundation (FAPESP) grants 14/21035-0, 16/07332-7, 13/08711-3, and 14/10068-4. CS was recipient of a CAPES-FAPERJ Postdoc fellowship. Other funds are provided by the National Council for Scientific and Technological Development (CNPq), the Instituto Nacional de Ciência e Tecnologia de Neurociência Translacional (INCT-INNT), Foundation for Research Support in the State of Rio de Janeiro (FAPERJ), Coordination for the Improvement of Higher Education Personnel (CAPES), Brazilian Funding Authority for Studies and Projects (FINEP), and Brazilian Development Bank (BNDES)pt_BR
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