Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/34299
Type: Artigo de periódico
Title: Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA
Author: Mehta, Angela
Rosato, Yoko B.
Abstract: Xanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X. axonopodis pv. citri. These genes were distributed in different functional categories, including genes that encode hypothetical proteins, genes involved in metabolism, cellular processes and pathogenicity, and mobile genetic elements. Most of these genes are likely related to growth and/or acquisition of nutrients in specific treatments whereas others might be important for the bacterium pathogenicity.
Subject: citrus canker
differential expression
leaf extract
in vivo
Editor: Sociedade Brasileira de Genética
Rights: aberto
Identifier DOI: 10.1590/S1415-47572005000100024
Address: http://dx.doi.org/10.1590/S1415-47572005000100024
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100024
Date Issue: 1-Mar-2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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