Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/342622
Type: Artigo
Title: Database establishment for the secondary fungal DNA barcode translational elongation factor 1 alpha (TEF1 alpha)
Author: Meyer, Wieland
Irinyi, Laszlo
Minh Thuy Vi Hoang
Robert, Vincent
Garcia-Hermoso, Dea
Desnos-Ollivier, Marie, Yurayart Chompoonek
Tsang Chi-Ching
Lee Chun-Yi
Woo, Patrick C. Y.
Pchelin, Ivan Mikhailovich
Uhrlass, Silke
Nenoff, Pietro
Chindamporn, Ariya
Chen Sharon
Hebert, Paul D. N.
Sorrell, Tania C.
Halliday, Catriona
Arthur, Ian
Moretti, Maria Luiza de Almeida
Soares, Celia Maria
Muniz, Mauro de Medeiros
Zancope-Oliveira, Rosely Maria
Cruz, Fundação Oswaldo
Melo, Analy Salles de Azevedo
Colombo, Arnaldo L.
Nishikaku, Angela Satie
Hendrickx, Marijke
Stubbe, Dirk
Normand, Anne-Cecile
Piarroux, Renaud
Ranque, Stephane
Dromer, Francoise
Arabatzis, Michael
Velegraki, Aristea
Cardinali, Gianluigi
Castanon, Laura Rosio
Taylor, Maria Lucia
Toriello, Conchita
de Hoog, Sybren
Pais, Celia
de Beer, Wilhelm
Gryzenhout, Marieka
Guarro, Josep
Cano-Lira, Jose F.
Robbertse, Barbara
Abstract: With new or emerging fungal infections, human and animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately identify pathogens from pure cultures. DNA barcodes are easily amplified, universal, short species-specific DNA sequences, which enable rapid identification by comparison with a well-curated reference sequence collection. The primary fungal DNA barcode, ITS region, was introduced in 2012 and is now routinely used in diagnostic laboratories. However, the ITS region only accurately identifies around 75% of all medically relevant fungal species, which has prompted the development of a secondary barcode to increase the resolution power and suitability of DNA barcoding for fungal disease diagnostics. The translational elongation factor 1 alpha (TEF1 alpha) was selected in 2015 as a secondary fungal DNA barcode, but it has not been implemented into practice, due to the absence of a reference database. Here, we have established a quality-controlled reference database for the secondary barcode that together with the ISHAM-ITS database, forms the ISHAM barcode database, available online at http://its.mycologylab.org/. We encourage the mycology community for active contributions
Subject: Fungos
DNA
Country: Canada
Editor: Canadian Science Publishing
Rights: Fechado
Identifier DOI: 10.1139/gen-2018-0083
Address: https://www.nrcresearchpress.com/doi/10.1139/gen-2018-0083
Date Issue: 2019
Appears in Collections:FCM - Artigos e Outros Documentos

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