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Type: Artigo
Title: Disruption of membrane integrity by the bacterium-derived antifungal jagaricin
Author: Fischer, Daniel
Gessner, Guido
Fill, Taicia Pacheco
Barnett, Robert
Tron, Kyrylo
Dornblut, Katharina
Kloss, Florian
Stallforth, Pierre
Hube, Bernhard
Heinemann, Stefan H.
Hertweck, Christian
Scherlach, Kirstin
Brunke, Sascha
Abstract: Jagaricin is a lipopeptide produced by the bacterial mushroom pathogen Janthinobacterium agaricidamnosum, the causative agent of mushroom soft rot disease. Apart from causing lesions in mushrooms, jagaricin is a potent antifungal active against human-pathogenic fungi. We show that jagaricin acts by impairing membrane integrity, resulting in a rapid flux of ions, including Ca2+, into susceptible target cells. Accordingly, the calcineurin pathway is required for jagaricin tolerance in the fungal pathogen Candida albicans. Transcriptional profiling of pathogenic yeasts further revealed that jagaricin triggers cell wall strengthening, general shutdown of membrane potential-driven transport, and the upregulation of lipid transporters, linking cell envelope integrity to jagaricin action and resistance. Whereas jagaricin shows hemolytic effects, it exhibited either no or low plant toxicity at concentrations at which the growth of prevalent phytopathogenic fungi is inhibited. Therefore, jagaricin may have potential for agricultural applications. The action of jagaricin as a membrane-disrupting antifungal is promising but would require modifications for use in humans
Subject: Candida albicans
Country: Estados Unidos
Editor: American Society for Microbiology
Rights: Fechado
Identifier DOI: 10.1128/AAC.00707-19
Date Issue: 2019
Appears in Collections:IQ - Artigos e Outros Documentos

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