Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/342438
Type: Artigo
Title: Characterization of RHD alleles present in serologically RHD-negative donors determined by a sensitive microplate technique
Author: de Paula Vendrame, Tatiane Aparecida
Arnoni, Carine Prisco
Muniz, Janaina Guilhem
Person, Rosangela de Medeiros
Pereira Cortez, Afonso Jose
Moreira Latini, Flavia Roche
Castilho, Lilian
Abstract: Weak D phenotypes with very low antigen densities and DEL phenotype may not be detected in RhD typing routine and could be typed as D-negative, leading to D alloimmunization of D-negative recipients. The present study aimed to investigate the presence of RHD-positive genotypes in blood donors typed as D-negative by an automated system using the solid-phase methodology as a confirmatory test. Two screenings were performed in different selected donor populations. For the first screening, we selected 1403 blood donor samples typed as D-negative regardless of the CE status, and in the second screening, we selected 517 donor samples typed as D-negative C+ and/or E+. RhD typing was performed by microplate in an automated equipment (Neo-Immucor (R)), and the confirmatory test was performed by solid-phase technique using Capture R (R) technology. A multiplex PCR specific to RHD and RHD psi was performed in a pool of 6 DNA samples. Sequencing of RHD exons was performed in all RHD-positive samples, and a specific PCR was used to identify the D-CE(4-7)-D hybrid gene. No weak D type was found in either screening populations. Additionally, 353 (18 center dot 4%) D-negative samples presented previously reported non-functional RHD genes, 2 samples had a DEL allele, and 6 samples demonstrated new alleles, including one novel DEL allele. Our study identified six new RHD alleles and showed that the inclusion of a confirmatory test using serological methodology with high sensitivity can reduce the frequency of weak D samples typed as D-negative
Subject: Grupos sanguíneos
Country: Suiça
Editor: Wiley
Rights: Fechado
Identifier DOI: 10.1111/vox.12851
Address: https://onlinelibrary.wiley.com/doi/full/10.1111/vox.12851
Date Issue: 2019
Appears in Collections:HEMO - Artigos e Outros Documentos

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