Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/341845
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampJardim, Isabel Cristina Sales Fontes-
dc.typeArtigopt_BR
dc.titleModified QuEChERS method for phenolic compounds determination in mustard greens (brassica juncea) using UHPLC-MS/MSpt_BR
dc.contributor.authorNicácio, Antonio Eduardo-
dc.contributor.authorRodrigues, Carina Alexandra-
dc.contributor.authorJardim, Isabel Cristina Sales Fontes-
dc.contributor.authorVisentainer, Jesuí Vergilio-
dc.contributor.authorMaldaner, Liane-
dc.subjectAntioxidantespt_BR
dc.subject.otherlanguageAntioxidantspt_BR
dc.description.abstractA modified QuEChERS method (Quick, Easy, Cheap, Effective, Rugged, and Safe) for the determination of fifteen phenolic compounds in mustard greens (Brassica juncea) using ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) analysis was developed. The QuEChERS partitioning step and dispersive solid phase extraction (d-SPE) clean-up sorbents were investigated, aimed at phenolic compound extraction and pigment removal, respectively. QuEChERS acetate version combined with 25 mg of diatomaceous earth (DE) and 5.0 mg of graphitized carbon black (GCB) provided the best conditions for sample preparation of the target compounds. Under the optimized conditions, all phenolic compounds showed good linearity (r ≥ 0.99) over the concentration range of 0.1 to 8000 μg kg−1, and the quantification limits were in the range of 0.06–230 μg kg−1. The spectrophotometric analysis showed that the clean-up step promoted a significant removal of chlorophyll, which is the major pigment present in the sample. Furthermore, antioxidant activity analysis was also carried out after the clean-up step and, together with chromatographic data, showed that no significant retention of the phenolic compounds occurs in the clean-up step. Two mustard greens varieties – Southern Giant Curled (SGC) and Florida Broadleaf (FB) - were analyzed with the proposed method. Seven phenolic compounds (4-hydroxybenzoic, p-coumaric, ferulic and sinapic acids, naringenin, apigenin and kaempferol) were found in both varieties, the greatest abundance being for sinapic acid (1261.5 ± 23 μg kg−1 in SGC and 1235.5 ± 26 μg kg−1 in FB) and ferulic acid (2861 ± 24 μg kg−1 in SGC and 3204.5 ± 45 μg kg−1 in FB)pt_BR
dc.relation.ispartofArabian journal of chemistrypt_BR
dc.publisher.cityAmsterdam pt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevierpt_BR
dc.date.issued2020-
dc.date.monthofcirculationMar.pt_BR
dc.language.isoengpt_BR
dc.description.volume13pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage4681pt_BR
dc.description.lastpage4690pt_BR
dc.rightsAbertopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn1878-5352pt_BR
dc.identifier.eissn1878-5379pt_BR
dc.identifier.doi10.1016/j.arabjc.2019.10.012pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1878535219301327pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO DO ESTADO DO PARANÁ - FApt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.date.available2020-05-20T21:01:29Z-
dc.date.accessioned2020-05-20T21:01:29Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-05-20T21:01:29Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:15:25Z : No. of bitstreams: 1 2-s2.0-85075855617.pdf: 1216575 bytes, checksum: 06aa3242d9ebbaa13806795cad804e4f (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-20T21:01:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2020en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/341845-
dc.contributor.departmentDepartamento de Química Analíticapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordQuEChERSpt_BR
dc.subject.keywordMustard greenspt_BR
dc.subject.keywordClean-uppt_BR
dc.subject.keywordSorbentspt_BR
dc.subject.keywordd-SPEpt_BR
dc.identifier.source2-s2.0-85075855617pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigo originalpt_BR
Appears in Collections:IQ - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
2-s2.0-85075855617.pdf1.19 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.