Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/341827
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampDuran Caballero, Nelson Eduardo-
dc.contributor.authorunicampCosta, Amanda Ferreira-
dc.contributor.authorunicampStanisic, Danijela-
dc.contributor.authorunicampBernardes, Juliana da Silva-
dc.contributor.authorunicampTasic, Ljubica-
dc.typeArtigopt_BR
dc.titleNanotoxicity and dermal application of nanostructured lipid carrier loaded with hesperidin from orange residuept_BR
dc.contributor.authorDurán, Nelson-
dc.contributor.authorCosta, Amanda F.-
dc.contributor.authorStanisic, Danijela-
dc.contributor.authorBernardes, Juliana S.-
dc.contributor.authorTasic, Ljubica T-
dc.subjectMateriais nanoestruturadospt_BR
dc.subjectFlavonóidespt_BR
dc.subjectMelanomapt_BR
dc.subject.otherlanguageNanostructured materialspt_BR
dc.subject.otherlanguageFlavonoidspt_BR
dc.subject.otherlanguageMelanomapt_BR
dc.description.abstractFlavonoids are a class of polyphenolic compounds that can be extracted from citrus fruits. Because of their features, they have been considered as new bullets in cancer therapy. Flavonoids present anti-oxidant, anti-inflammatory and anti-cancer properties and some studies are pointing that their higher intake involves lower risks of cancer. The nanostructured lipid carriers (NLC) are drug delivery systems composed from a mixture of solid and liquid lipids that increase physical stability and promote the sustained release of the drug. So, the main aim of this research was to combine the potential of the flavonoid as a natural anti-oxidant compound with the nanostructured lipid carriers to form an efficient system for flavonoid delivery into the cells. These NLC were prepared through high pressure homogenization technique and were evaluated in function of several standard properties. The samples exhibited good stability through time and temperature, with sizes of around 215 nm and zeta potentials of -35 mV during a period of 105 days. The encapsulation and the loading efficiency were respectively 96% and 2.25% up to 105 days of storage. In vitro release assays showed a burst process for NLC-L pointing to their shell structure with flavonoid situated in the contour of the particles. The cell viability tests showed that flavonoid, either free or loaded into NLC, did not present in vitro toxicity to the melanoma cells tested: A375, CHL01 and SKMEL147 through 3-(4,5-dimethyiltiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red and crystal violet assays. Hesperidin at 45 μmol L−1 was able to reduce significantly the radical forming up to 92.58 ± 0.82% in DPPH assay. On the other hand, the produced NLC were applied in a skin lotion formulation, which showed a good stability and opened up a new application for the produced nanoparticles as in anti-aging and moisturizing cosmetics. Finally, the in vitro cytotoxicity tests were carried out using several human culture cells. The flavonoid loaded and empty nanocarriers have been used to treat the cells. All data suggested that, at the level studied, non-significant toxicities on cells were observedpt_BR
dc.relation.ispartofJournal of physics : conference seriespt_BR
dc.relation.ispartofabbreviationJPCSpt_BR
dc.publisher.cityBristolpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherIOP Publishingpt_BR
dc.date.issued2019-
dc.date.monthofcirculationOct.pt_BR
dc.language.isoengpt_BR
dc.description.volume1323pt_BR
dc.rightsAbertopt_BR
dc.sourceSCOPUSpt_BR
dc.identifier.issn1742-6588pt_BR
dc.identifier.eissn1742-6596pt_BR
dc.identifier.doi10.1088/1742-6596/1323/1/012021pt_BR
dc.identifier.urlhttps://iopscience.iop.org/article/10.1088/1742-6596/1323/1/012021pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.date.available2020-05-20T18:44:37Z-
dc.date.accessioned2020-05-20T18:44:37Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-05-20T18:44:37Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:15:18Z : No. of bitstreams: 1 2-s2.0-85074936935.pdf: 314862 bytes, checksum: 1045523bbc196d8d10c368b9ec1db97f (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-20T18:44:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/341827-
dc.contributor.departmentDepartamento de Química Orgânicapt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Química Orgânicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordCytologypt_BR
dc.identifier.source2-s2.0-85074936935pt_BR
dc.creator.orcid0000-0001-8372-5143pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0001-8830-5973pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-2930-7332pt_BR
dc.type.formArtigopt_BR
dc.identifier.articleid012021pt_BR
dc.description.sponsorNoteCAPES, CNPq, FAEPEX, FAPESP, LNNano and NanoBioss are kindly acknowledged. Thanks to Prof. R. Chammas (ICESP) for help with the initial cytotoxicity assayspt_BR
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