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Type: Artigo
Title: Genes controlled by DNA methylation are involved in wilms tumor progression
Author: da Silva Guerra, Joao Victor
de Sa Pereira, Bruna Maria
Vieira da Cruz, Jessica Goncalves
Scherer, Nicole de Miranda
Furtado, Carolina
de Azevedo, Rafaela Montalvao
Lopes de Oliveira, Paulo Sergio
Faria, Paulo
Boroni, Mariana
de Camargo, Beatriz
Maschietto, Mariana
Abstract: To identify underlying mechanisms involved with metastasis formation in Wilms tumors (WTs), we performed comprehensive DNA methylation and gene expression analyses of matched normal kidney (NK), WT blastemal component, and metastatic tissues (MT) from patients treated under SIOP 2001 protocol. A linear Bayesian framework model identified 497 differentially methylated positions (DMPs) between groups that discriminated NK from WT, but MT samples were divided in two groups. Accordingly, methylation variance grouped NK and three MT samples tightly together and all WT with four MT samples that showed high variability. WT were hypomethylated compared to NK, and MT had a hypermethylated pattern compared to both groups. The methylation patterns were in agreement with methylases and demethylases expression. Methylation data pointed to the existence of two groups of metastases. While hierarchical clustering analysis based on the expression of all 2569 differentially expressed genes (DEGs) discriminated WT and MT from all NK samples, the hierarchical clustering based on the expression of 44 genes with a differentially methylated region (DMR) located in their promoter region revealed two groups: one containing all NKs and three MTs and one containing all WT and four MTs. Methylation changes might be controlling expression of genes associated with WT progression. The 44 genes are candidates to be further explored as a signature for metastasis formation in WT
Subject: Nefroblastoma
Metilação de DNA
Country: Suiça
Editor: MDPI
Rights: Aberto
Identifier DOI: 10.3390/cells8080921
Date Issue: 2019
Appears in Collections:IB - Artigos e Outros Documentos

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