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Type: Artigo
Title: Biomass accumulation and cell wall structure of rice plants overexpressing a dirigent-jacalin of sugarcane (ShDJ) under varying conditions of water availability
Author: Andrade, Larissa Mara
Peixoto-Junior, Rafael Favero
Ribeiro, Rafael Vasconcelos
Nobile, Paula Macedo
Brito, Michael Santos
Ribeiro Marchiori, Paulo Eduardo
Carlin, Samira Domingues
Boer Martins, Alexandre Palma
Goldman, Maria Helena S.
Portilla Llerena, Juan Pablo
Fregonesi, Caroline
Perecin, Dilermando
Carlos de Oliveira Nebo, Joao Felipe
Figueira, Antonio
Benatti, Thiago Romanos
da Silva, Jorge
Mazzafera, Paulo
Abstract: A sugarcane gene encoding a dirigent-jacalin, ShDJ, was induced under drought stress. To elucidate its biological function, we integrated a ShDJ-overexpression construction into the rice Nipponbare genome via Agrobacterium- mediated transformation. Two transgenic lines with a single copy gene in T-0 were selected and evaluated in both the T-1 and T-4 generations. Transgenic lines had drastically improved survival rate under water deficit conditions, at rates close to 100%, while WT did not survive. Besides, transgenic lines had improved biomass production and higher tillering under water deficit conditions compared with WT plants. Reduced pectin and hemicellulose contents were observed in transgenic lines compared with wild-type plants under both well-watered and water deficit conditions, whereas cellulose content was unchanged in line #17 and reduced in line #29 under conditions of low water availability. Changes in lignin content under water deficit were only observed in line #17. However, improvements in saccharification were found in both transgenic lines along with changes in the expression of OsNTS1/2 and OsMYB58/63 secondary cell wall biosynthesis genes. ShDJ-overexpression upregulated the expression of the OsbZIP23, OsGRAS23, OsP5CS, and OsLea3 genes in rice stems under well-watered conditions. Taken together, our data suggest that ShDJ has the potential for improving drought tolerance, plant biomass accumulation, and saccharification efficiency
Subject: Plantas transgênicas
Superexpressão gênica
Country: Suiça
Editor: Frontiers Research Foundation
Rights: Aberto
Identifier DOI: 10.3389/fpls.2019.00065
Date Issue: 2019
Appears in Collections:FCM - Artigos e Outros Documentos

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