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http://repositorio.unicamp.br/jspui/handle/REPOSIP/341728
Type: | Artigo |
Title: | Long-term methylglyoxal intake aggravates murine Th2-mediated airway eosinophil infiltration |
Author: | Medeiros, M.L. de Oliveira, M.G. avares, E.G. Mello, G.C. Anhê, G.F. Mónica, F.Z. Antunes, E. |
Abstract: | Asthma outcomes is aggravated in obese patients. Excess of methylglyoxal (MGO) in obese/diabetic patients has been associated with diverse detrimental effects on cell function. This study aimed to evaluate the effects of long-term oral intake of MGO on ovalbumin-induced eosinophil inflammation. Male C57/Bl6 mice received 0.5% MGO in the drinking water for 12 weeks. Mice were sensitized and challenged with ovalbumin (OVA), and at 48 h thereafter, bronchoalveolar lavage (BAL) fluid and lungs were collected for cell counting, morphological analysis, and ELISA, mRNA expressions and DHE assays. In MGO-treated mice, OVA challenge significantly increased the peribronchiolar infiltrations of inflammatory cells and eosinophils compared with control group. Higher levels of IL-4, IL-5, and eotaxin in BAL fluid were also detected in MGO compared with control group. In addition, lung tissue of MGO-treated mice displayed significant increases in mRNA expressions of NF-κB and iNOS whereas COX-2 expression remained unchanged. The high TNF-α mRNA expression observed in lungs of OVA-challenged control mice was not further increased by MGO treatment. In MGO group, OVA-challenge increased significantly the NOX-2 and NOX-4 mRNA expressions, without affecting the NOX-1 expression. Levels of reactive-oxygen species (ROS) were significantly higher in lungs of MGO-treated mice, and no further increase by OVA-challenge was observed. In conclusion, 12-week intake of MGO exacerbates Th2-mediated airway eosinophil infiltration by activation of NF-kB/iNOS-dependent signaling pathway and positive regulation of NOX-2 and NOX-4 in the lung tissues. Scavengers of MGO could be an option to prevent obesity-related asthma |
Subject: | NADPH oxidase |
Country: | Reino Unido |
Editor: | Elsevier |
Rights: | Fechado |
Identifier DOI: | 10.1016/j.intimp.2020.106254 |
Address: | https://www.sciencedirect.com/science/article/pii/S1567576919327778 |
Date Issue: | 2020 |
Appears in Collections: | FCM - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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2-s2.0-85078456135.pdf | 3.27 MB | Adobe PDF | View/Open |
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